TGFBR2/TGF-beta RII 蛋白, Human (HEK293, mFc-Avi)

The TGFBR2/TGF-β RII protein cooperates with TGFBR1 to form dedicated receptors for TGFB1, TGFB2, and TGFB3. It serves as a signal transducer that coordinates diverse physiological and pathological processes, including cell cycle regulation, mesenchymal cell dynamics, wound healing, matrix production, immunosuppression, and carcinogenesis. TGFBR2/TGF-beta RII Protein, Human (HEK293, mFc-Avi) is the recombinant human-derived TGFBR2/TGF-beta RII protein, expressed by HEK293 , with C-Avi, C-mFc labeled tag. The total length of TGFBR2/TGF-beta RII Protein, Human (HEK293, mFc-Avi) is 136 a.a., with molecular weight of 55-70 kDa.

跨膜丝氨酸/苏氨酸激酶 TGFBR2 (TGF-β RII) 与 TGF-β I 型丝氨酸/苏氨酸激酶受体 TGFBR1 协作，形成 TGF-β 细胞因子的专用受体，包括 TGFB1、TGFB2 和 TGFB3。TGFBR2 作为信号转导器，介导 TGFB1、TGFB2 和 TGFB3 信号从细胞表面到细胞质的传递，从而协调多种生理和病理过程。这些包括上皮细胞和造血细胞的细胞周期停滞、间充质细胞增殖和分化的调节、伤口愈合、细胞外基质的产生、免疫抑制和致癌作用。该受体复合物包含与细胞因子二聚体对称结合的 2 个 TGFBR1 和 2 个 TGFBR2 分子，导致 TGFBR1 被组成型活性 TGFBR2 磷酸化和激活。激活的 TGFBR1 随后磷酸化 SMAD2，导致其与受体解离并与 SMAD4 相互作用。由此产生的 SMAD2-SMAD4 复合物易位到细胞核，在细胞核中调节 TGF-β 调节基因的转录，构成典型的 SMAD 依赖性 TGF-β 信号级联。此外，TGFBR2 参与非经典、不依赖 SMAD 的 TGF-β 信号传导途径，并表现出转化生长因子 β 激活受体活性。

1. Immobilized Human Mature TGF beta 1, No Tag at 0.5 μg/mL (100 μl/well) on the plate. Dose response curve for Human TGF-beta RII, mFc Tag with the EC₅₀ of 8 ng/mL determined by ELISA.
2. Immobilized Human TGF-beta RII, mFc Tag at 0.5 μg/mL (100 μl/well) on the plate. Dose response curve for Biotinylated Human Mature TGF beta 3, Avi Tag with the EC₅₀ of 14.6 ng/mL determined by ELISA.

Human

HEK293

C-Avi;C-mFc

P37173 (I24-D159)

7048  [NCBI]

55-70 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.