TGM2/Transglutaminase 2 蛋白, Mouse (HEK293, C-His)

The TGM2/transglutaminase 2 protein is a calcium-dependent acyltransferase involved in bone development, angiogenesis, wound healing, and apoptosis.TGM2 is also involved in neurotransmission-independent actions and serves as a signal transducer in α-1 adrenergic receptor-mediated pathways.TGM2/Transglutaminase 2 Protein, Mouse (HEK293, C-His) is the recombinant mouse-derived TGM2/Transglutaminase 2 protein, expressed by HEK293 , with C-10*His labeled tag.

TGM2/转谷氨酰胺酶 2 蛋白是一种钙依赖性酰基转移酶，催化肽结合的谷氨酰胺和各种伯胺之间形成共价键，导致蛋白质交联或胺化。TGM2 充当蛋白质-谷氨酰胺 γ-谷氨酰转移酶，参与多种生物过程，如骨骼发育、血管生成、伤口愈合、细胞分化、染色质修饰和细胞凋亡。它介导蛋白质的交联，包括 ACO2、HSPB6、FN1、HMGB1、RAP1GDS1、SLC25A4/ANT1、SPP1 和 WDR54。在生理条件下，GTP 会抑制蛋白质交联活性，而在应对各种应激时，Ca(2+) 会缓解这种抑制。除了通过促进细胞骨架蛋白交联和介导细胞外基质支架的形成在细胞凋亡中发挥作用外，TGM2 还可以使用单胺底物催化多种蛋白质翻译后修饰。它通过介导组蛋白 H3 的血清素化和多巴胺化在染色质组织中发挥至关重要的作用，有助于腹侧被盖区 (VTA) 神经元的神经传递独立功能。此外，TGM2 在 α1 肾上腺素受体介导的磷脂酶 C-δ (PLCD) 活性刺激中充当信号转导器，突出显示其参与除酰基转移酶活性之外的信号通路。

Measured by its ability to form CBZ-Gln-Gly-Hydroxamate from CBZ-Gln-Gly and Hydroxylamine that incubate at 37°C for 2 h. The specific activity is 2765.799 pmol/min/μg.

Mouse

HEK293

C-10*His

P21981 (A2-A686)

21817

AEELLLERCDLEIQANGRDHHTADLCQEKLVLRRGQRFRLTLYFEGRGYEASVDSLTFGAVTGPDPSEEAGTKARFSLSDNVEEGSWSASVLDQQDNVLSLQLCTPANAPIGLYRLSLEASTGYQGSSFVLGHFILLYNAWCPADDVYLDSEEERREYVLTQQGFIYQGSVKFIKSVPWNFGQFEDGILDTCLMLLDMNPKFLKNRSRDCSRRSSPIYVGRVVSAMVNCNDDQGVLLGRWDNNYGDGISPMAWIGSVDILRRWKEHGCQQVKYGQCWVFAAVACTVLRCLGIPTRVVTNYNSAHDQNSNLLIEYFRNEFGELESNKSEMIWNFHCWVESWMTRPDLQPGYEGWQAIDPTPQEKSEGTYCCGPVSVRAIKEGDLSTKYDAPFVFAEVNADVVDWIRQEDGSVLKSINRSLVVGQKISTKSVGRDDREDITHTYKYPEGSPEEREVFTKANHLNKLAEKEETGVAMRIRVGDSMSMGNDFDVFAHIGNDTSETRECRLLLCARTVSYNGVLGPECGTEDINLTLDPYSENSIPLRILYEKYSGCLTESNLIKVRGLLIEPAANSYLLAERDLYLENPEIKIRVLGEPKQNRKLVAEVSLKNPLSDPLYDCIFTVEGAGLTKEQKSVEVSDPVPAGDLVKARVDLFPTDIGLHKLVVNFQCDKLKSVKGYRNVIIGPA

Approximately 70-90 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.