TGS1 蛋白, Human (His-SUMO)

TGS1 protein catalyzes the conversion of the 7-monomethylguanosine (m(7)G) cap of small nuclear RNA (snRNA) and small nucleolar RNA (snoRNA) into 2, 2,7-trimethylguanosine (m( 2,2,7)G) Cap structure. The enzyme shows specificity for guanine, with N7 methylation occurring before N2 methylation during the modification process. TGS1 Protein, Human (His-SUMO) is the recombinant human-derived TGS1 protein, expressed by E. coli , with N-His, N-SUMO labeled tag. The total length of TGS1 Protein, Human (His-SUMO) is 141 a.a., with molecular weight of ~31.6 kDa.

TGS1 或三甲基鸟苷合酶 1 是一种关键酶，可催化两个连续的甲基化步骤，这些步骤对于将小核 RNA (snRNA) 和小核仁 RNA (snoRNA) 上存在的 7-单甲基鸟苷 (m7G) 帽转化为 2,2、 7-三甲基鸟苷 (m2,2,7G) 帽结构。该过程涉及鸟嘌呤在 N7 位点的特异性甲基化，然后是 N2 甲基化。U snRNA 上 m7G 帽的高甲基化导致它们集中在核灶中，与线圈蛋白共定位，并形成典型的卡哈尔小体 (CB)。TGS1 参与这些过程表明它在转录调节和核结构动态组织中的作用。它必须强调 TGS1 对鸟嘌呤的特异性及其在塑造 RNA 分子帽结构、影响与 RNA 修饰和定位相关的细胞过程中的关键功能。

Human

E. coli

N-His;N-SUMO

Q96RS0 (713M-853T)

96764  [NCBI]

MRVIAIDIDPVKIALARNNAEVYGIADKIEFICGDFLLLASFLKADVVFLSPPWGGPDYATAETFDIRTMMSPDGFEIFRLSKKITNNIVYFLPRNADIDQVASLAGPGGQVEIEQNFLNNKLKTITAYFGDLIRRPASET

Approximately 31.6 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.