Thrombomodulin 蛋白, Human (HEK293, His)

Thrombomodulin, an endothelial cell receptor, forms a 1:1 complex with thrombin, vital for coagulation regulation. This complex activates protein C, converting it to protein Ca. Protein Ca then inhibits the activated cofactors (factor Va and factor VIIIa) of the coagulation cascade, suppressing thrombin generation. Thrombomodulin's interaction with ITGAL, ITGAM, and ITGB2 emphasizes its role in immune response modulation. Thrombomodulin Protein, Human (HEK293, His) is the recombinant human-derived Thrombomodulin protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Thrombomodulin Protein, Human (HEK293, His) is 497 a.a., with molecular weight of ~64-67 kDa.

血栓调节蛋白是一种独特的内皮细胞受体，与凝血酶建立精确的 1:1 化学计量复合物，在凝血调节中发挥着至关重要的作用。该复合物促进蛋白 C 转化为其活化形式蛋白 Ca。一旦被激活，蛋白 Ca 通过裂解凝血级联的激活辅助因子（即因子 Va 和因子 VIIIa）发挥作用，有效抑制凝血酶的产生。血栓调节蛋白与 ITGAL、ITGAM 和 ITGB2 的相互作用进一步强调了其参与调节免疫反应。

Measured by its ability to mediate thrombin activation of protein C. The specific activity is 49380.98 pmol/min/µg, as measured under the described conditions.

Human

HEK293

C-6*His

P07204 (A19-S515)

7056

APAEPQPGGSQCVEHDCFALYPGPATFLNASQICDGLRGHLMTVRSSVAADVISLLLNGDGGVGRRRLWIGLQLPPGCGDPKRLGPLRGFQWVTGDNNTSYSRWARLDLNGAPLCGPLCVAVSAAEATVPSEPIWEEQQCEVKADGFLCEFHFPATCRPLAVEPGAAAAAVSITYGTPFAARGADFQALPVGSSAAVAPLGLQLMCTAPPGAVQGHWAREAPGAWDCSVENGGCEHACNAIPGAPRCQCPAGAALQADGRSCTASATQSCNDLCEHFCVPNPDQPGSYSCMCETGYRLAADQHRCEDVDDCILEPSPCPQRCVNTQGGFECHCYPNYDLVDGECVEPVDPCFRANCEYQCQPLNQTSYLCVCAEGFAPIPHEPHRCQMFCNQTACPADCDPNTQASCECPEGYILDDGFICTDIDECENGGFCSGVCHNLPGTFECICGPDSALARHIGTDCDSGKVDGGDSGSGEPPPSPTPGSTLTPPAVGLVHS

Approximately 64-67 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.