TIGIT 蛋白, Human (HEK293, Fc)

TIGIT Protein, Human (HEK293, Fc) produced in HEK293 cells with the C-termimal human IgG1 Fc fragment. T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is also known as V-set and immunoglobulin domain-containing protein 9 (VSIG9), V-set and transmembrane domain-containing protein 3 (VSTM3). It belongs to single-pass type I membrane protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM).

细胞 Ig 和 ITIM 结构域 (TIGIT) 是一种跨膜糖蛋白，属于 1 型脊髓灰质炎病毒受体家族蛋白，也与 CD155 和 CD112 配体结合。TIGIT 在外周记忆和调节性 CD4+ T 细胞以及 NK 细胞上表达，并在初始 CD4+ T 细胞上激活后上调。CD155 是单核细胞和 CD11c+ 人树突状细胞 (DC) 上表达的 TIGIT 的高亲和力受体。TIGIT 在其胞质结构域中包含一个 Ig 样 V 型结构域和一个 ITIM，表明受体占据可能会触发负信号传导事件^[1]。

5 µg/mL (100 µL/well) of immoblized recombinant human CD155-Fc can bind human Biotin-TIGIT-Fc with a linear range of 6.1-48.83 ng/mL.

Human

HEK293

C-hFc

Q495A1-1 (M22-P141)

201633  [NCBI]

MMTGTIETTGNISAEKGGSIILQCHLSSTTAQVTQVNWEQQDQLLAICNADLGWHISPSFKDRVAPGPGLGLTLQSLTVNDTGEYFCIYHTYPDGTYTGRIFLEVLESSVAEHGARFQIP

50-55 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against PBS, 5% trehalose and mannitol.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Lozano E, et al. The TIGIT/CD226 axis regulates human T cell function. J Immunol. 2012 Apr 15;188(8):3869-75.  [Content Brief]