TIM-3/HAVCR2 蛋白, Human (Biotinylated, HEK293, His-Avi)

The TIM-3/HAVCR2 protein regulates immune responses to pathogens, interacting with LGALS9 on infected macrophages to limit bacterial growth. It enhances CD8+ T cell responses and acts as a phosphatidylserine receptor, mediating phagocytosis of apoptotic cells. TIM-3/HAVCR2 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived TIM-3/HAVCR2 protein, expressed by HEK293 , with C-Avi, C-His labeled tag.

所描述的蛋白质参与对细胞内病原体的先天免疫反应。它与结核分枝杆菌感染的巨噬细胞上的 LGALS9 相互作用，刺激抗菌活性并限制细菌生长。然而，这种蛋白作为 LGALS9 受体的作用受到了挑战。它还增强 CD8+ T 细胞对急性感染的反应，并充当钙依赖性磷脂酰丝氨酸 (PtSer) 的受体。它可以识别凋亡细胞上的 PtSer 并介导其吞噬作用。该蛋白在 T 细胞和树突状细胞上表达，促进凋亡细胞的结合，但不促进其吞噬。它积极调节树突状细胞的先天免疫反应，并与 Toll 样受体协同作用，促进 TNF-α 的分泌。在肿瘤浸润树突状细胞中，它通过与 HMGB1 相互作用来抑制核酸介导的先天免疫反应。它作为自然杀伤细胞的辅助受体，增强响应 LGALS9 的 IFN-γ 产生，同时也抑制 NK 细胞介导的细胞毒性。它在 LPS 诱导的内毒素休克中负向调节 NK 细胞功能。该蛋白与多种其他蛋白相互作用，包括 HMGB1、BAG6、PIK3R1、PIK3R2、FYN、VAV1、AKT1/2、LCP2、ZAP70、SYK、SH3BP2、SH2D2A、LCK、PLCG 和 ILF3。它与 ILF3 的相互作用促进 ILF3 泛素化和降解。

Immobilized Anti-Tim-3 Antibody, hFc Tag at 1 μg/mL (100 μl/well) on the plate. Dose response curve for Biotinylated Human Tim-3, His Tag with the EC₅₀ of 15.8 ng/mL determined by ELISA.

Human

HEK293

C-Avi;C-His

Q8TDQ0-1 (S22-R200)

84868  [NCBI]

SEVEYRAEVGQNAYLPCFYTPAAPGNLVPVCWGKGACPVFECGNVVLRTDERDVNYWTSRYWLNGDFRKGDVSLTIENVTLADSGIYCCRIQIPGIMNDEKFNLKLVIKPAKVTPAPTRQRDFTAAFPRMLTTRGHGPAETQTLGSLPDINLTQISTLANELRDSRLANDLRDSGATIR

45-50 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.