TLE3 蛋白, Human (GST)

The TLE3 protein is a transcriptional corepressor that inhibits Wnt signaling by binding to transcription factors such as CTNNB1 and TCF family members. It forms homotetramers, hetero-oligomers, and interacts with AES. TLE3 Protein, Human (GST) is the recombinant human-derived TLE3 protein, expressed by E. coli , with N-GST labeled tag. The total length of TLE3 Protein, Human (GST) is 289 a.a., with molecular weight of ~58.5 kDa.

TLE3 是一种转录辅阻遏物，通过与多种转录因子结合并对 Wnt 信号通路中 CTNNB1 和 TCF 家族成员介导的转录激活发挥抑制作用，复杂地调节基因表达。TLE3 与其他家族成员形成同源四聚体和异源寡聚体，其调节影响可能通过其与显性失活因子 AES 的关联来调节。在其分子相互作用中，TLE3 与 LEF1、TCF7 和 TCF7L1 结合，确定其参与调节这些关键因子的转录活性。此外，TLE3 还与 FOXA2、XIAP/BIRC4 和 TCF7L2/TCF4 发生蛋白质间相互作用。值得注意的是，它与 TBX18 的相互作用，涉及嵌入的同源 1 阻遏基序，导致 TBX18 转录活性降低，强调了 TLE3 在微调特定转录反应中的作用。

Human

E. coli

N-His

Q04726 (S484-Y772)

7090  [NCBI]

SHGEVVCAVTISNPTRHVYTGGKGCVKIWDISQPGSKSPISQLDCLNRDNYIRSCKLLPDGRTLIVGGEASTLTIWDLASPTPRIKAELTSSAPACYALAISPDAKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGASCIDISHDGTKLWTGGLDNTVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHHTKPDKYQLHLHESCVLSLKFAYCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISADDKYIVTGSGDKKATVYEVIY

Approximately 32 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCL, 150 mM NaCl, 5 mM GSH, pH 7.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.