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  3. TMED1 蛋白, Mouse (HEK293, Fc)

TMED1 蛋白, Mouse (HEK293, Fc)

目录号: HY-P77241
COA 产品使用指南

TMED1(即跨膜 p24 运输蛋白 1)是 EMP24/GP25L 家族的成员。TMED1 蛋白, Mouse (HEK293, Fc) 是重组的 TMED1 蛋白,由 HEK293 表达,带有 C-hFc 标签。TMED1 蛋白, Mouse (HEK293, Fc) 全长 170 个氨基酸,分子量约为 ~56-60 KDa。

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规格 价格 是否有货 数量
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5 μg ¥680 In-stock
10 μg ¥1080 In-stock
50 μg ¥2800 In-stock
100 μg ¥4500 In-stock
500 μg ¥12600 询价
> 500 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

TMED1, or Transmembrane p24 trafficking protein 1, is a member of the EMP24/GP25L family. TMED1 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived TMED1 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of TMED1 Protein, Mouse (HEK293, Fc) is 170 a.a., with molecular weight of ~56-60 KDa.

研究背景

TMED1 蛋白属于 TMED(含跨膜 emp24 结构域)蛋白家族,参与蛋白的囊泡运输。TMED1 蛋白通过其与白细胞介素 1 受体样 1 (IL1RL1) 的相互作用而被鉴定,并且可能在先天免疫中发挥作用。TMED1 蛋白与其他白细胞介素 1 配体缺乏任何相似性。

生物活性

Measured by its binding ability in a functional ELISA. Immobilized Mouse TMED1 at 0.5 μg/mL (100 μL/well) can bind Human ST2. The ED50 for this effect is 2.39 μg/mL.

  • Measured by its binding ability in a functional ELISA. Immobilized Mouse TMED1 at 0.5 μg/mL (100 μL/well) can bind Human ST2. The ED50 for this effect is 2.39 μg/mL.
种属

Mouse

表达系统

HEK293

标签

C-hFc

蛋白编号

B2RRM5/NP_034874.2 (G25-N194)

基因 ID
蛋白结构
N-term
TMED1 (G25-N194)
Accession # B2RRM5/NP_034874.2
hFc
C-term
同用名
Transmembrane emp24 domain-containing protein 1; Interleukin-1 receptor-like 1 ligand; Tp24; IL1RL1L; IL1RL1LG
氨基酸序列

GPPPIQDGEFTFLLPAGRKQCFYQSAPANASLETEYQVIGGAGLDVDFTLESPQGVLLVSESRKADGVHTVEPTEAGDYRLCFDNSFSTISEKLVFFELIFDSFQDEEEVEGWAEAVEPEEMLDVKMEDIKESIETMRTRLERSIQMLTLLRAFEARDRNLQEDNLERVN

分子量

Approximately 56-60 kDa due to the glycosylation.

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

TMED1 Protein, Mouse (HEK293, Fc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
TMED1 Protein, Mouse (HEK293, Fc)
目录号:
HY-P77241
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