TMPRSS11D 蛋白, Human (HEK293, His)

The TMPRSS11D protein is associated with host mucosal defense and acts synergistically with substances in the airway mucosa. It critically processes ACE2, activating the human coronavirus 229E spike glycoprotein to achieve virus-cell membrane fusion. TMPRSS11D Protein, Human (HEK293, His) is the recombinant human-derived TMPRSS11D protein, expressed by HEK293 , with N-His labeled tag. The total length of TMPRSS11D Protein, Human (HEK293, His) is 232 a.a., with molecular weight of ~27.6 kDa.

TMPRSS11D 蛋白独立地或与气道粘液或支气管分泌物中的其他物质协同作用，在粘膜宿主防御系统中发挥潜在的生物学作用。值得注意的是，它在 ACE2 的蛋白水解过程中发挥着关键作用，有助于激活人类冠状病毒 229E (HCoV-229E) 刺突糖蛋白。这种激活促进病毒与细胞膜的融合，这是病毒进入的关键步骤。TMPRSS11D 对刺突糖蛋白的蛋白水解活性涉及在特定肽的 P1 位置处裂解精氨酸残基的 C 端侧，其中 Boc-Phe-Ser-Arg-4-methylcoumaryl-7-amide 是最有效的裂解底物。使用该底物时，其酶活性在 pH 值为 8.6 时达到最佳。该蛋白被二异丙基氟磷酸盐、亮抑酶肽、抗痛药、抑肽酶和大豆胰蛋白酶抑制剂强烈抑制，而分泌性白细胞蛋白酶抑制剂在 10 µM 时显示出最小的抑制作用。这些发现表明，TMPRSS11D 在宿主防御和病毒进入过程中发挥多方面的作用，突出了其在调节粘膜免疫和病毒感染中的潜在重要性。

Human

HEK293

N-His

O60235/NP_004253.1 (I187-I418)

9407  [NCBI]

ILGGTEAEEGSWPWQVSLRLNNAHHCGGSLINNMWILTAAHCFRSNSNPRDWIATSGISTTFPKLRMRVRNILIHNNYKSATHENDIALVRLENSVTFTKDIHSVCLPAATQNIPPGSTAYVTGWGAQEYAGHTVPELRQGQVRIISNDVCNAPHSYNGAILSGMLCAGVPQGGVDACQGDSGGPLVQEDSRRLWFIVGIVSWGDQCGLPDKPGVYTRVTAYLDWIRQQTGI

Approximately 27.6 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.