TMPRSS2 蛋白, Human (P.pastoris, His)

The TMPRSS2 protein is a plasma membrane-anchored serine protease that plays critical roles in prostate physiology, cancer metastasis, pain modulation, and viral infection. Its lytic activity affects proteolytic cascades involved in prostate function, and androgen-induced activation contributes to cancer progression. TMPRSS2 Protein, Human (P.pastoris, His) is the recombinant human-derived TMPRSS2 protein, expressed by P. pastoris , with N-6*His labeled tag. The total length of TMPRSS2 Protein, Human (P.pastoris, His) is 387 a.a., with molecular weight of ~45 kDa.

TMPRSS2 蛋白是一种质膜锚定的丝氨酸蛋白酶，在各种生理和病理过程中发挥着独特的作用。TMPRSS2 以其精氨酸残基的裂解活性而闻名，它在对前列腺正常生理功能至关重要的蛋白水解级联中发挥着关键作用。在前列腺癌中，雄激素诱导的 TMPRSS2 激活导致前肝细胞生长因子/HGF、蛋白酶激活受体 2/F2RL1 和 matriptase/ST14 等底物裂解，促进细胞外基质破坏和转移。此外，TMPRSS2 通过激活三叉神经元来调节疼痛敏感性，影响自发性疼痛和机械性异常性疼痛。在微生物感染领域，TMPRSS2 通过两种独立的机制，在促进人类冠状病毒 SARS-CoV 和 SARS-CoV-2 的感染中发挥着关键作用：ACE2 受体的蛋白水解裂解，促进病毒摄取，以及冠状病毒刺突的裂解糖蛋白，激活糖蛋白进入宿主细胞。这种蛋白酶对于甲型流感病毒的传播和发病机制也至关重要，参与血凝素 (HA) 蛋白的蛋白水解裂解和激活，而血凝素 (HA) 蛋白是病毒感染性所必需的。TMPRSS2 的多种功能强调了其在正常生理过程和疾病发病机制中的重要性。

Recombinant Human TMPRSS2 His tag protein enzyme activity is measured by its ability to cleave fluorogenic peptide substrate(Boc-Gln-Ala-Arg-AMC).

Human

P. pastoris

N-6*His

O15393 (W106-G492)

7113  [NCBI]

WKFMGSKCSNSGIECDSSGTCINPSNWCDGVSHCPGGEDENRCVRLYGPNFILQVYSSQRKSWHPVCQDDWNENYGRAACRDMGYKNNFYSSQGIVDDSGSTSFMKLNTSAGNVDIYKKLYHSDACSSKAVVSLRCIACGVNLNSSRQSRIVGGESALPGAWPWQVSLHVQNVHVCGGSIITPEWIVTAAHCVEKPLNNPWHWTAFAGILRQSFMFYGAGYQVEKVISHPNYDSKTKNNDIALMKLQKPLTFNDLVKPVCLPNPGMMLQPEQLCWISGWGATEEKGKTSEVLNAAKVLLIETQRCNSRYVYDNLITPAMICAGFLQGNVDSCQGDSGGPLVTSKNNIWWLIGDTSWGSGCAKAYRPGVYGNVMVFTDWIYRQMRADG

Approximately 45 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.