TMX1 蛋白, Human (HEK293, Fc)

TMX1 Protein is a member of the protein disulfide isomerase (PDI) family and is a transmembrane thiol isomerase. TMX1 Protein can participate in various redox reactions, reversibly oxidizes to disulfide through its active center thiol, and catalyzes the thiol-disulfide exchange reaction. In addition, TMX1 Protein negatively regulates the activation of αibβ3 integrin and platelet aggregation outside the cell. TMX1 Protein, Human (HEK293, Fc) is the recombinant human-derived TMX1 protein, expressed by HEK293 , with C-mFc labeled tag. The total length of TMX1 Protein, Human (HEK293, Fc) is 154 a.a., with molecular weight of 45-50 kDa.

TMX1 Protein 可参与各种氧化还原反应，既可以氧化二硫键，也可以还原二硫键，并选择性地作用于膜相关多肽^[1]。
TMX1 Protein 作为巯基肿瘤抑制因子，可以增加线粒体 ATP 的产生和凋亡的进展。TMX1 Protein 在氧化条件下以硫醇依赖的方式与 SERCA2b 相互作用，从而降低 SERCA 活性，从而降低内质网 Ca2+ 负荷^[2]。
细胞外的 TMX1 Protein 对 αibβ3 整合素的激活和血小板聚集有负调控作用^[3]。

Human

HEK293

C-mFc

AAH36460 (R27-S180)

81542  [NCBI]

RRSNVRVITDENWRELLEGDWMIEFYAPWCPACQNLQPEWESFAEWGEDLEVNIAKVDVTEQPGLSGRFIINALPTIYHCKDGEFRRYQGPRTKKDFINFISDKEWKSIEPVSSWFGPGSVLMSSMSALFQLSMWIRTCHNYFIEDLGLPVWGS

Approximately 45-50 kDa due to the glycosylation.

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.