1. 重组蛋白
  2. Cytokines and Growth Factors
  3. TNF Superfamily Neurotrophic Factors
  4. TNF Superfamily Ligands
  5. TNF-alpha
  6. TNF-alpha/TNFSF2 蛋白, Mouse (His-SUMO)

TNF-alpha/TNFSF2 蛋白, Mouse (His-SUMO)

目录号: HY-P700510
产品使用指南

TNF-α/TNFSF2 蛋白由巨噬细胞分泌,与 TNFRSF1A/TNFR1 和 TNFRSF1B/TNFBR 结合,诱导肿瘤细胞死亡并充当热原。它与恶病质有关,刺激细胞增殖和分化,并诱导脂肪细胞中的胰岛素抵抗,从而导致 TNF 诱导的胰岛素抵抗。TNF-alpha/TNFSF2 蛋白, Mouse (His-SUMO) 是重组的 TNF-α/TNFSF2 蛋白,由 E. coli 表达,带有 N-SUMO, N-6*His 标签。

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描述

TNF-α/TNFSF2 protein is secreted by macrophages, binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR, induces tumor cell death and acts as a pyrogen.It is associated with cachexia, stimulates cell proliferation and differentiation, and induces insulin resistance in adipocytes, leading to TNF-induced insulin resistance.TNF-alpha/TNFSF2 Protein, Mouse (His-SUMO) is the recombinant mouse-derived TNF-alpha/TNFSF2 protein, expressed by E.coli , with N-SUMO, N-6*His labeled tag.

研究背景

TNF-α/TNFSF2 蛋白是一种与 TNFRSF1A/TNFR1 和 TNFRSF1B/TNFBR 结合的细胞因子。主要由巨噬细胞分泌,可诱导某些肿瘤细胞系的细胞死亡。此外,它是一种强热原,通过直接作用或刺激白细胞介素-1 分泌而引起发烧,并与恶病质的诱发有关。在特定条件下,能刺激细胞增殖,促进细胞分化。在脂肪细胞中,它通过抑制胰岛素诱导的 IRS1 酪氨酸磷酸化和葡萄糖摄取来诱导胰岛素抵抗,同时还会导致 GKAP42 蛋白降解,从而导致 TNF 诱导的胰岛素抵抗。TNF-α/TNFSF2 蛋白通过与 IL1B 和 IL6 协同诱导 VEGF 产生,在血管生成中发挥作用。此外,它促进破骨细胞生成,从而介导骨吸收。最后,TNF-α/TNFSF2 蛋白的 TNF 胞内结构域 (ICD) 形式可刺激树突状细胞中 IL12 的产生。

种属

Mouse

表达系统

E. coli

标签

N-SUMO;N-6*His

蛋白编号

P06804 (G57-L235)

基因 ID
蛋白结构
N-term
6*His-SUMO
TGF-α (G57-L235)
Accession # P06804
C-term
同用名
rHuTNF-α, His; Cachectin; TNFSF2
氨基酸序列

GPQRDEKFPNGLPLISSMAQTLTLRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLVYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPKDTPEGAELKPWYEPIYLGGVFQLEKGDQLSAEVNLPKYLDFAESGQVYFGVIAL

分子量

35.7 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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