RANK/TNFRSF11A 蛋白, Human (His)

RANK (TNFRSF11A) is a receptor activator of nuclear factor κB (NF-κB), which is involved in bone metabolism and immune system function. RANK is mainly expressed by osteoblasts/stromal cells, and is associated with the formation of giant cell tumor of bone (GCTBs) ^[1]. RANK combined with RANKL ligand activated the RANKL/RANK/ OPG system to regulate bone resorption ^[2]. RANKL/RANK is also a regulator of dendritic cell (DC)-T cell interaction, which is associated with mammary epithelial formation and central nervous system thermoregulation in lactating female ^[3][4]. RANK/TNFRSF11A Protein, Human (His) has 175 amino acids (28L-202K), produced by HEK293 cells with C-terminal His-tag.

RANK (TNFRSF11A) 是核因子 κB (NF-κB) 的受体激活剂，最初被描述为在骨代谢和免疫系统中发挥关键作用。RANK属于肿瘤坏死因子受体超家族，在破骨细胞分化和活化过程中发挥作用。RANK由成骨细胞/基质细胞表达，在骨骼肌、胸腺、肝脏、结肠、小肠和肾上腺中普遍表达且高水平。然而，成骨细胞通常大量存在于骨巨细胞瘤 (GCTB) 中，这表明肿瘤中表达因子会刺激破骨细胞前体募集和分化^[1]。RANK与NF-κB配体的受体激活剂RANKL结合，触发RANKL/RANK/骨保护素（OPG）系统来调节骨吸收。具体来说，RANKL/RANK信号通路促进多细胞破骨细胞前体的形成，保证多细胞破骨细胞在正常骨重塑和各种病理条件下的激活和存活。OPG 通过与 RANKL 竞争性结合并阻碍 RANK 与 RANKL 结合^[2]来保护骨骼免受过度骨吸收。除了 RANK 对骨代谢的贡献外，RANKL/RANK 系统还参与树突状细胞 (DC)-T 细胞相互作用。在类风湿滑膜和淋巴结对中，RANK 和 RANKL 的表达水平可作为判断树突状细胞与 T 细胞相互作用的标志物^[3]。此外，RANKL-RANK系统对于哺乳期女性乳腺上皮的形成和中枢神经系统的体温调节至关重要。由于 RANKL-RANK 受到女性性激素雌二醇和黄体酮的严格控制，当循环中的女性性激素减少时，RANKL-RANK 会导致绝经后妇女骨质疏松症。此外，RANKL-RANK 信号转导在其他骨病理、骨转移或激素驱动的乳腺癌中也发挥着关键作用^[4]。

Human

E. coli

N-His

Q9Y6Q6 (28L-202K)

8792  [NCBI]

LQIAPPCTSEKHYEHLGRCCNKCEPGKYMSSKCTTTSDSVCLPCGPDEYLDSWNEEDKCLLHKVCDTGKALVAVVAGNSTTPRRCACTAGYHWSQDCECCRRNTECAPGLGAQHPLQLNKDTVCKPCLAGYFSDAFSSTDKCRPWTNCTFLGKRVEHHGTEKSDAVCSSSLPARK

Approximately 23.2 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.

• [1]. Roux S, et al. RANK (receptor activator of nuclear factor kappa B) and RANK ligand are expressed in giant cell tumors of bone. Am J Clin Pathol. 2002 Feb;117(2):210-6.  [Content Brief]

  [2]. Boyce BF, et al. Biology of RANK, RANKL, and osteoprotegerin. Arthritis Res Ther. 2007;9 Suppl 1(Suppl 1):S1.  [Content Brief]

  [3]. Page G, et al. RANK and RANKL expression as markers of dendritic cell-T cell interactions in paired samples of rheumatoid synovium and lymph nodes. Arthritis Rheum. 2005 Aug;52(8):2307-12.  [Content Brief]

  [4]. Nagy V, et al. The RANKL-RANK Story. Gerontology. 2015;61(6):534-42.  [Content Brief]