TNFSF3/Lymphotoxin Beta 蛋白, Cynomolgus (sf9, His)

Lymphotoxin Beta (LT-beta) also known as TNFSF3, is a type II transmembrane glycoprotein of the TNF family. Lymphotoxin Beta binds to LTα to form membrane-anchored heterotrimers that activate canonical/noncanonical nuclear factor-κB (NF-κB) signaling thus inducing chemokine, cytokine or adhesion molecule expression, cell proliferation and cell survival. Lymphotoxin Beta also shows anti-inflammatory, anti-cancer activity^[1]. Lymphotoxin Beta regulates hepatic stellate cell function and wound healing in a murine model of chronic liver injury^[2]. TNFSF3/Lymphotoxin Beta Protein, Cynomolgus (sf9, His) is a recombinant protein with a N-Terminal His label, It consists of 197 amino acids (Q49-G244) and is produced in Sf9 insect cells.

LT 由 T、B、自然杀伤 (NK) 和淋巴组织诱导细胞表达。而淋巴毒素 Beta 在炎性细胞、LPC 以及邻近纤维间隔的偶尔小肝细胞上表达^[1][2]。
氨基酸序列人淋巴毒素β蛋白与小鼠淋巴毒素β蛋白同源性较低。而人淋巴毒素 Beta 与恒河猴淋巴毒素 Beta 蛋白具有 96.72% 的氨基酸序列同一性。
淋巴毒素-β (LTβ) 作为 II 型跨膜蛋白专门锚定在膜上，与 LTα 结合形成膜锚定异源三聚体 (LTα1β2)和 LTα2β1)。LTα1β2 触发 LTβR，而据报道 LTα2β1 结合 TNFR1 和 TNFR2，LIGHT (TNFSF14) 是 LTβR 的替代配体。此外，LTαβ 异源三聚体可激活经典/非经典核因子-κB (NF-κB) 信号传导，这是先天性和适应性免疫反应、细胞存活或凋亡、细胞应激反应、淋巴器官发育和维护的重要调节因子^[1] 。
淋巴毒素-β (LTβ) 是肿瘤坏死因子 (TNF) 家族的促炎细胞因子。Lymphotoxin-β 激活 NF-κB 信号传导并具有抗炎、抗癌活性^[1]。Lymphotoxin-β 会增加肝星状细胞中 IκBα mRNA 和蛋白质的表达，并调节慢性肝损伤小鼠模型中的肝星状细胞功能和伤口愈合^[2]。Lymphotoxin-β 可促进促炎细胞因子 TNFα 和 IL-1β 的 mRNA 表达，增强 RelA 的 mRNA 表达，可能是膀胱癌的潜在治疗靶点^[3]。

Lymphotoxin-β (mouse) (-1 ng/mL; 12 h) increases the expression of IκBα mRNA, an NF-κB-responsive gene in a time and dose dependent manner, and increases the protein levels of IκBα in hepatic stellate cells (HSCs)^[2].

Cynomolgus

Sf9 insect cells

N-His

XP_005553605.2 (Q49-G244)

101013049

Approximately 22.8 kDa.

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.

• [1]. Wolf MJ, et al. The unexpected role of lymphotoxin beta receptor signaling in carcinogenesis: from lymphoid tissue formation to liver and prostate cancer development. Oncogene. 2010 Sep 9;29(36):5006-18.  [Content Brief]

  [2]. Ruddell RG, et al. Lymphotoxin-beta receptor signaling regulates hepatic stellate cell function and wound healing in a murine model of chronic liver injury. Hepatology. 2009 Jan;49(1):227-39.  [Content Brief]

  [3]. Shen M, et al. Lymphotoxin β receptor activation promotes mRNA expression of RelA and pro-inflammatory cytokines TNFα and IL-1β in bladder cancer cells. Mol Med Rep. 2017 Jul;16(1):937-942.  [Content Brief]