TPM4 蛋白, Human (HEK293, His)

TPM4 protein, binding actin filaments in muscle and non-muscle cells, crucially regulates calcium-dependent muscle contraction, aided by the troponin complex in vertebrates. In smooth muscle cells, TPM4 interacts with caldesmon, contributing to contraction regulation. In non-muscle cells, it stabilizes actin filaments in the cytoskeleton. TPM4's calcium-binding ability, homodimer, and heterodimer formation (with alpha and beta chains) showcase its versatile molecular configurations. TPM4's multifaceted interactions emphasize its essential roles in muscle contraction and cytoskeletal dynamics across diverse cellular contexts. TPM4 Protein, Human (HEK293, His) is the recombinant human-derived TPM4 protein, expressed by HEK293, with N-His, N-6*His labeled tag. The total length of TPM4 Protein, Human (HEK293, His) is 247 a.a., with molecular weight of 35-40 kDa.

TPM4 蛋白对肌肉和非肌肉细胞中的肌动蛋白丝表现出结合特异性，在脊椎动物横纹肌收缩的钙依赖性调节中发挥着关键作用，特别是与肌钙蛋白复合物结合。在平滑肌细胞中，TPM4 通过与 caldesmon 相互作用来调节收缩。在非肌肉细胞中，TPM4 参与稳定细胞骨架内的肌动蛋白丝。此外，研究表明，TPM4 具有钙结合能力。它形成同二聚体和异二聚体，后者由 α 链（TPM1、TPM3 或 TPM4）和 β 链（TPM2）组成，展示了其分子构型的多功能性。TPM4 的多方面相互作用强调了它在不同细胞环境中的肌肉收缩和细胞骨架动力学中的重要作用。

Human

HEK293

N-His;N-6*His

P67936 (A2-I248)

7171  [NCBI]

AGLNSLEAVKRKIQALQQQADEAEDRAQGLQRELDGERERREKAEGDVAALNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRAMKDEEKMEIQEMQLKEAKHIAEEADRKYEEVARKLVILEGELERAEERAEVSELKCGDLEEELKNVTNNLKSLEAASEKYSEKEDKYEEEIKLLSDKLKEAETRAEFAERTVAKLEKTIDDLEEKLAQAKEENVGLHQTLDQTLNELNCI

35-40 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS, pH 7.4. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.