TPO/Thrombopoietin 蛋白, Mouse (335a.a, HEK293, C-His)

TPO/Thrombopoietin Protein, a lineage-specific cytokine, crucially regulates megakaryocyte proliferation and maturation, acting in the late stages of development from committed progenitor cells. Recognized as a major physiological regulator, TPO significantly influences circulating platelets, emphasizing its importance in the intricate process of platelet formation. TPO/Thrombopoietin Protein, Mouse (HEK293, C-His) is the recombinant mouse-derived TPO/Thrombopoietin protein, expressed by HEK293 , with C-6*His labeled tag.

血小板生成素 (TPO) 是一种谱系特异性细胞因子，在调节巨核细胞的增殖和成熟中发挥着至关重要的作用，在定向祖细胞发育的后期发挥作用。TPO 作为巨核细胞生成的关键因子，被认为是循环血小板的主要生理调节剂。它对巨核细胞发育后期的影响强调了它在血小板形成的复杂过程中的重要性。

Measured  in a cell proliferation assay using MO7e human megakaryocytic leukemic cells.  The ED₅₀ for this effect is 0.6746 ng/mL, corresponding to a specific  activity is 1.482×10⁶ units/mg.

Mouse

HEK293

C-6*His

P40226-1 (S22-T356)

21832  [NCBI]

SPVAPACDPRLLNKLLRDSHLLHSRLSQCPDVDPLSIPVLLPAVDFSLGEWKTQTEQSKAQDILGAVSLLLEGVMAARGQLEPSCLSSLLGQLSGQVRLLLGALQGLLGTQLPLQGRTTAHKDPNALFLSLQQLLRGKVRFLLLVEGPTLCVRRTLPTTAVPSSTSQLLTLNKFPNRTSGLLETNFSVTARTAGPGLLSRLQGFRVKITPGQLNQTSRSPVQISGYLNRTHGPVNGTHGLFAGTSLQTLEASDISPGAFNKGSLAFNLQGGLPPSPSLAPDGHTPFPPSPALPTTHGSPPQLHPLFPDPSTTMPNSTAPHPVTMYPHPRNLSQET

approximately 75 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 2 mM EDTA, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.