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  4. TPP2 蛋白, Human (Myc, His)

TPP2 蛋白, Human (Myc, His)

目录号: HY-P71451
产品使用指南

TPP2 蛋白是一种胞质三肽基肽酶，是泛素蛋白酶体途径的重要组成部分，在 26S 蛋白酶体下游发挥作用。它通过从多肽中释放N端三肽，在维持细胞内氨基酸稳态中发挥着至关重要的作用。此外，TPP2 蛋白参与刺激脂肪生成，有助于脂肪组织形成的过程。TPP2 蛋白, Human (Myc, His) 是重组的 TPP2 蛋白，由 E. coli 表达，带有 N-His, C-Myc 标签。TPP2 蛋白, Human (Myc, His) 全长 221 个氨基酸，分子量约为 ~31.8 kDa。

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描述

TPP2 protein, a cytosolic tripeptidyl-peptidase, is a vital component of the ubiquitin-proteasome pathway, operating downstream of the 26S proteasome. It plays a crucial role in maintaining intracellular amino acid homeostasis by releasing N-terminal tripeptides from polypeptides. Furthermore, TPP2 protein is implicated in stimulating adipogenesis, contributing to processes involved in adipose tissue formation. TPP2 Protein, Human (Myc, His) is the recombinant human-derived TPP2 protein, expressed by E. coli , with N-His, C-Myc labeled tag. The total length of TPP2 Protein, Human (Myc, His) is 221 a.a., with molecular weight of ~31.8 kDa.
研究背景

胞质三肽基肽酶 TPP2 蛋白是泛素蛋白酶体途径中蛋白水解级联的关键组成部分，作用于 26S 蛋白酶体的下游。它在细胞内氨基酸稳态中发挥着至关重要的作用，具有从多肽中释放 N 末端三肽的作用。此外，TPP2 蛋白与刺激脂肪生成有关，有助于参与脂肪组织形成的细胞过程。
种属

Human
表达系统

E. coli
标签

N-His;C-Myc
蛋白编号

P29144 (44D-264H)
基因 ID
蛋白结构
N-term
10*His
TPP2 (44D-264H)
Accession # P29144
Myc
C-term
同用名
TPP2; Tripeptidyl-peptidase 2; TPP-2; EC 3.4.14.10; Tripeptidyl aminopeptidase; Tripeptidyl-peptidase II; TPP-II
氨基酸序列

DTGVDPGAPGMQVTTDGKPKIVDIIDTTGSGDVNTATEVEPKDGEIVGLSGRVLKIPASWTNPSGKYHIGIKNGYDFYPKALKERIQKERKEKIWDPVHRVALAEACRKQEEFDVANNGSSQANKLIKEELQSQVELLNSFEKKYSDPGPVYDCLVWHDGEVWRACIDSNEDGDLSKSTVLRNYKEAQEYGSFGTAEMLNYSVNIYDDGNLLSIVTSGGAH

分子量

Approximately 31.8 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

TPP2 Protein, Human (Myc, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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