TRADD 蛋白, Human (His)

The TRADD protein acts as a linker for TNFRSF1A/TNFR1 and mediates its interaction with FADD. TRADD induces TNF-induced responses: apoptosis and NF-kappa-B activation. TRADD Protein, Human (His) is the recombinant human-derived TRADD protein, expressed by E. coli , with C-6*His labeled tag.

TRADD 蛋白作为 TNFRSF1A/TNFR1 的衔接分子，与激活的 TNFRSF1A/TNFR1 的细胞质结构域特异性结合，介导其与 FADD 的相互作用。TRADD 的过度表达会诱导 TNF 诱导的两种主要反应：细胞凋亡和 NF-κ-B 的激活。TRADD 的核形式通过与 TRIP12 相互作用，阻止 CDKN2A 的同种型 p19ARF/ARF 泛素化和降解，从而破坏 TRIP12 与 CDKN2A 的同种型 p19ARF/ARF 之间的相互作用，从而起到肿瘤抑制剂的作用。TNF-α 受体 TNFRSF1A 的刺激会导致两种不同信号复合物的形成。质膜结合复合物 I 由 TNFRSF1A、TRADD、RIPK1、TRAF2 和 BIRC2/c-IAP1 或 BIRC3 组成，与 CHUCK/IKK-alpha、IKBKB/IKK-beta 和 IKBKG/IKK-gamma 相互作用，促进细胞存活。随后，TRADD、RIPK1和TRAF2与TNFRSF1A解离，与FADD和caspase CASP8形成细胞质复合物II，促进细胞凋亡。TRADD 还与这些复合物中的各种蛋白质相互作用，包括 TRPC4AP、UXT1、DAB2IP、SQSTM1、TRIP12、HIPK2、KRT14、KRT18、KRT16、KRT17、FADD 和 TOMM70，突出了其在不同细胞过程中的多方面作用。

Human

E. coli

C-6*His

Q15628 (M1-A312)

8717

Greater than 90 % as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.