TRAILR4/TNFRSF10D 蛋白, Human (HEK293, His)

TRAILR4/TNFRSF10D protein is a receptor for TRAIL and lacks the ability to induce apoptosis due to the truncated death domain. Paradoxically, not only does it fail to induce apoptosis, but it also prevents TRAIL-mediated apoptosis. TRAILR4/TNFRSF10D Protein, Human (HEK293, His) is the recombinant human-derived TRAILR4/TNFRSF10D protein, expressed by HEK293 , with C-His labeled tag.

TRAILR4/TNFRSF10D 蛋白作为细胞毒性配体 TRAIL 的受体发挥作用，尽管它包含截短的死亡结构域，使其无法诱导细胞凋亡。矛盾的是，TRAILR4/TNFRSF10D 不仅不能诱导细胞凋亡，而且还对 TRAIL 介导的细胞凋亡发挥保护作用。关于其激活 NF-kappa-B 通路的能力存在相互矛盾的信息，一些研究表明它不能诱导该通路，而其他研究则认为它具有激活 NF-kappa-B 的能力。TRAILR4/TNFRSF10D 与 TRAIL 相互作用的双重性质，既作为受体又作为抗凋亡的保护因子，强调了其在细胞对 TRAIL 信号传导反应中调节功能的复杂性。

Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L-929 mouse fibroblast cells treated with TRAIL. The ED₅₀ for this effect is 20.99 ng/mL in the presence of 20 ng/mL of rhTRAIL, corresponding to a specific activity is 4.76 ×10⁴ units/mg.

Human

HEK293

C-6*His

Q9UBN6 (A56-H211)

8793  [NCBI]

ATIPRQDEVPQQTVAPQQQRRSLKEEECPAGSHRSEYTGACNPCTEGVDYTIASNNLPSCLLCTVCKSGQTNKSSCTTTRDTVCQCEKGSFQDKNSPEMCRTCRTGCPRGMVKVSNCTPRSDIKCKNESAASSTGKTPAAEETVTTILGMLASPYH

30-40 kDa

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.