TRAILR4/TNFRSF10D 蛋白, Rhesus Macaque (HEK293, His)

TRAILR4/TNFRSF10D Protein , a receptor for TRAIL. Paradoxically, it not only fails to induce apoptosis but also protects against TRAIL-mediated apoptosis. Conflicting reports exist about its role in activating the NF-kappa-B pathway. The dual nature of TRAILR4 in interacting with TRAIL, both as a receptor and a protective factor, highlights the complexity of its regulatory functions in cellular responses to TRAIL signaling. TRAILR4/TNFRSF10D Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived TRAILR4/TNFRSF10D protein, expressed by HEK293 , with C-His labeled tag.

TRAILR4/TNFRSF10D 作为细胞毒性配体 TRAIL 的受体发挥作用，由于它包含截短的死亡结构域，使其无法诱导细胞凋亡。矛盾的是，TRAILR4 不仅不能诱导细胞凋亡，而且还对 TRAIL 介导的细胞凋亡发挥保护作用。关于其激活 NF-kappa-B 通路的能力存在相互矛盾的信息，一些研究表明它不能诱导该通路，而其他研究则认为它具有激活 NF-kappa-B 的能力。TRAILR4 与 TRAIL 相互作用的双重性质，既作为受体，又作为抗凋亡的保护因子，强调了其在细胞对 TRAIL 信号传导反应中调节功能的复杂性^[1][2][3]。

Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L-929 mouse fibroblast cells treated with TRAIL. The ED₅₀ for this effect is 6.265 ng/mL in the presence of 20 ng/mL of rhTRAIL, corresponding to a specific activity is 1.60 ×10⁵ units/mg.

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  Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L-929 mouse fibroblast cells treated with TRAIL. The ED₅₀ for this effect is 6.265 ng/mL in the presence of 20 ng/mL of rhTRAIL, corresponding to a specific activity is 1.60 ×10⁵ units/mg.

Rhesus Macaque

HEK293

C-6*His

XP_001107922 (A56-L206)

716901  [NCBI]

ATISRKDEVPLPPVASKQQRRSLEAECPAGFHRSEHTGACNPCTEGVDYTIASNNLPSCLLCTVCKSGQTNTSSCTTTRDTVCQCEKGSFQDANSPEMCRKCSTGCPRGMIKVSDCTPWSDINCSASAASSTGKTPAAEDTVPTSLGTPDL

Approximately 22-32 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.