Transferrin 蛋白, Pig (HEK293, His)

Transferrin is an important iron-binding transport protein that binds two Fe(3+) ions, usually with anions such as bicarbonate. Crucially, it transports iron from the site of absorption and heme degradation to the site of storage and utilization. Transferrin Protein, Pig (HEK293, His) is the recombinant pig-derived Transferrin protein, expressed by HEK293 , with C-His labeled tag. The total length of Transferrin Protein, Pig (HEK293, His) is 694 a.a., with molecular weight of ~80 kDa.

转铁蛋白作为铁结合转运蛋白，表现出结合两个 Fe(3+) 离子的能力，通常与阴离子（通常是碳酸氢根）的结合结合。从功能上讲，转铁蛋白在将铁从吸收和血红素降解部位转运到体内专门储存和利用的部位方面发挥着至关重要的作用。除了主要参与铁稳态之外，血清转铁蛋白还可能在刺激细胞增殖方面发挥额外作用。转铁蛋白的结构为单体，其多功能性强调了其在协调铁转运和细胞调节的复杂过程中的重要性，有助于生物体内的基本生理功能。

Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED₅₀ for this effect is 22.35 ng/mL, corresponding to a specific activity is 4.47×10⁴ units/mg.

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  Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED₅₀ for this effect is 22.35 ng/mL, corresponding to a specific activity is 4.47×10⁴ units/mg.

Pig

HEK293

C-His

B3CL06 (Q22-T715)

396996  [NCBI]

QKTVRWCTISNQEANKCSSFRENMSKAVKNGPLVSCVKKSSYLDCIKAIRDKEADAVTLDAGLVFEAGLAPYNLKPVVAEFYGQKDNPQTHYYAVAVVKKGSNFQWNQLQGKRSCHTGLGRSAGWIIPMGLLYDQLPEPRKPIEKAVASFFSSSCVPCADPVNFPKLCQQCAGKGAEKCACSNHEPYFGYAGAFNCLKEDAGDVAFVKHSTVLENLPDKADRDQYELLCRDNTRRPVDDYENCYLAQVPSHAVVARSVDGQEDSIWELLNQAQEHFGRDKSPDFQLFSSSHGKDLLFKDSANGFLRIPSKMDSSLYLGYQYVTALRNLREEISPDSSKNECKKVRWCAIGHEETQKCDAWSINSGGKIECVSAENTEDCIAKIVKGEADAMSLDGGYIYIAGKCGLVPVLAENYKTEGENCVNTPEKGYLAVAVVKKSSGPDLNWNNLKGKKSCHTAVDRTAGWNIPMGLLYNKINSCKFDQFFGEGCAPGSQRNSSLCALCIGSERAPGRECLANNHERYYGYTGAFRCLVEKGDVAFVKDQVVQQNTDGKNKDDWAKDLKQMDFELLCQNGAREPVDNAENCHLARAPNHAVVARDDKVTCVAEELLKQQAQFGRHVTDCSSSFCMFKSNTKDLLFRDDTQCLARVGKTTYESYLGADYITAVANLRKCSTSKLLEACTFHSAKNPRVETTT

Approximately 80 kDa

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  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.