1. 重组蛋白
  2. Enzymes & Regulators Ubiquitin Related Proteins
  3. Transferases (EC 2) Ubiquitin Enzymes
  4. E3 Ligases
  5. TRIM/RBCC Proteins
  6. TRIM16 蛋白, Human (Sf9, His, Strep)

TRIM16 蛋白, Human (Sf9, His, Strep)

目录号: HY-P702067
产品使用指南

TRIM16蛋白是一种必需的E3泛素连接酶,参与溶酶体和吞噬体损伤过程中的自噬反应和泛素化。TRIM16 蛋白, Human (Sf9, His, Strep) 是重组的 TRIM16 蛋白,由 Sf9 insect cells 表达,带有 N-Strep, N-8*His 标签。TRIM16 蛋白, Human (Sf9, His, Strep) 全长 563 个氨基酸。

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     可免费申领三个不同产品的试用装。

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描述

TRIM16 protein is an essential E3 ubiquitin ligase that participates in autophagy reaction and ubiquitination during lysosome and phagosome damage. TRIM16 Protein, Human (Sf9, His, Strep) is the recombinant human-derived TRIM16 protein, expressed by Sf9 insect cells , with N-Strep, N-8*His labeled tag. The total length of TRIM16 Protein, Human (Sf9, His, Strep) is 563 a.a., .

研究背景

TRIM16 作为 E3 泛素连接酶,在面对溶酶体和吞噬体损伤时协调自噬反应和泛素化过程中发挥着至关重要的作用。值得注意的是,它通过复杂地调节 p62-KEAP1-NRF2 信号轴来促进应激诱导的蛋白质聚集体的生物发生和降解。其功能的核心在于调节泛素化水平,从而调节 NRF2 的稳定性,NRF2 是细胞应激反应的关键参与者。作为支架蛋白,TRIM16 促进蛋白质聚集体的自噬降解,与 p62/SQSTM、ATG16L1 和 LC3B/MAP1LC3B 形成相互作用。这种多方面的作用使 TRIM16 成为防止氧化应激诱导的细胞死亡的守护者,特别是在内膜损伤的反应中。

种属

Human

表达系统

Sf9 insect cells

标签

N-Strep;N-8*His

蛋白编号

O95361 (A2-P564)

基因 ID

/

蛋白结构
N-term
8*His-Strep
TRIM16 (A2-P564)
Accession # O95361
C-term
同用名
TRIM16; Tripartite motif-containing protein 16; E3 ubiquitin-protein ligase TRIM16; Estrogen-responsive B box protein
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 重组蛋白稀释计算器

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
TRIM16 Protein, Human (Sf9, His, Strep)
目录号:
HY-P702067
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