TRIM5 蛋白, Human (His, B2M)

The TRIM5 protein is a capsid-specific restriction factor that prevents non-host adapted retroviral infection. TRIM5 acts as a pattern recognition receptor that activates innate immune signaling by triggering E3 ubiquitin ligase activity upon binding to viral capsids. TRIM5 restricts various retroviruses and regulates autophagy. TRIM5 Protein, Human (His, B2M) is the recombinant human-derived TRIM5 protein, expressed by E. coli , with N-His, B2M labeled tag.

TRIM5是一种衣壳特异性限制因子，可有效防止非宿主适应逆转录病毒的感染。其抗病毒活性发生在病毒生命周期的早期，特别是在病毒进入之后但在逆转录之前。除了作为衣壳特异性限制因子的作用外，TRIM5 还充当模式识别受体，激活先天免疫信号以响应逆转录病毒衣壳晶格。与病毒衣壳结合后，TRIM5 触发其 E3 泛素连接酶活性。它与 UBE2V1-UBE2N 复合物结合，生成“Lys-63”连接的多聚泛素链，促进 MAP3K7/TAK1 复合物的自身磷酸化。激活的 MAP3K7/TAK1 复合物会诱导 NF-kappa-B 和 MAPK 反应性炎症基因，从而在受感染的细胞中激发先天免疫反应。TRIM5的多功能性延伸至限制各种逆转录病毒的感染，包括N-热带性鼠白血病病毒、马传染性贫血病毒、猕猴猿免疫缺陷病毒、猫免疫缺陷病毒和牛免疫缺陷病毒。此外，TRIM5通过激活自噬调节因子BECN1并作为选择性自噬受体，靶向HIV-1衣壳蛋白p24进行自噬降解，在自噬调节中发挥着至关重要的作用。

Human

E. coli

N-His;B2M

Q9C035-1 (M1-S493)

85363  [NCBI]

MASGILVNVKEEVTCPICLELLTQPLSLDCGHSFCQACLTANHKKSMLDKGESSCPVCRISYQPENIRPNRHVANIVEKLREVKLSPEGQKVDHCARHGEKLLLFCQEDGKVICWLCERSQEHRGHHTFLTEEVAREYQVKLQAALEMLRQKQQEAEELEADIREEKASWKTQIQYDKTNVLADFEQLRDILDWEESNELQNLEKEEEDILKSLTNSETEMVQQTQSLRELISDLEHRLQGSVMELLQGVDGVIKRTENVTLKKPETFPKNQRRVFRAPDLKGMLEVFRELTDVRRYWVDVTVAPNNISCAVISEDKRQVSSPKPQIIYGARGTRYQTFVNFNYCTGILGSQSITSGKHYWEVDVSKKTAWILGVCAGFQPDAMCNIEKNENYQPKYGYWVIGLEEGVKCSAFQDSSFHTPSVPFIVPLSVIICPDRVGVFLDYEACTVSFFNITNHGFLIYKFSHCSFSQPVFPYLNPRKCGVPMTLCSPSS

Approximately 70.3 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.