TRIM5 蛋白, Human (N-His)

The TRIM5 protein is a capsid-specific restriction factor that prevents non-host adapted retroviral infection. TRIM5 acts as a pattern recognition receptor that activates innate immune signaling by triggering E3 ubiquitin ligase activity upon binding to viral capsids. TRIM5 restricts various retroviruses and regulates autophagy. TRIM5 Protein, Human (N-His) is the recombinant human-derived TRIM5 protein, expressed by E. coli , with N-6*His labeled tag.

TRIM5是一种衣壳特异性限制因子，可有效防止非宿主适应逆转录病毒的感染。其抗病毒活性发生在病毒生命周期的早期，特别是在病毒进入之后但在逆转录之前。除了作为衣壳特异性限制因子的作用外，TRIM5 还充当模式识别受体，激活先天免疫信号以响应逆转录病毒衣壳晶格。与病毒衣壳结合后，TRIM5 触发其 E3 泛素连接酶活性。它与 UBE2V1-UBE2N 复合物结合，生成“Lys-63”连接的多聚泛素链，促进 MAP3K7/TAK1 复合物的自身磷酸化。激活的 MAP3K7/TAK1 复合物会诱导 NF-kappa-B 和 MAPK 反应性炎症基因，从而在受感染的细胞中激发先天免疫反应。TRIM5的多功能性延伸至限制各种逆转录病毒的感染，包括N-热带性鼠白血病病毒、马传染性贫血病毒、猕猴猿免疫缺陷病毒、猫免疫缺陷病毒和牛免疫缺陷病毒。此外，TRIM5通过激活自噬调节因子BECN1并作为选择性自噬受体，靶向HIV-1衣壳蛋白p24进行自噬降解，在自噬调节中发挥着至关重要的作用。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His

Q9C035-1 (M1-Q248)

85363  [NCBI]

MASGILVNVKEEVTCPICLELLTQPLSLDCGHSFCQACLTANHKKSMLDKGESSCPVCRISYQPENIRPNRHVANIVEKLREVKLSPEGQKVDHCARHGEKLLLFCQEDGKVICWLCERSQEHRGHHTFLTEEVAREYQVKLQAALEMLRQKQQEAEELEADIREEKASWKTQIQYDKTNVLADFEQLRDILDWEESNELQNLEKEEEDILKSLTNSETEMVQQTQSLRELISDLEHRLQGSVMELLQ

Approximately 30 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, 200 mM arginine, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.