TrkB 蛋白, Human (HEK293, His)

The TrkB protein is a key receptor tyrosine kinase that plays a key role in central and peripheral nervous system development. It regulates neuronal survival, proliferation, migration, differentiation, synapse formation, and plasticity. TrkB Protein, Human (HEK293, His) is the recombinant human-derived TrkB protein, expressed by HEK293 , with C-6*His labeled tag.

TrkB 蛋白是一种受体酪氨酸激酶，在中枢和周围神经系统的发育和成熟中发挥着关键作用。它调节各种过程，包括神经元存活、增殖、迁移、分化、突触形成和可塑性。TrkB 作为 BDNF、NTF4 的受体，也具有结合 NTF3 的能力，但效率较低。配体结合后，TrkB 发生同二聚化、自磷酸化和激活。这会激活下游效应器，例如 SHC1、FRS2、SH2B1、SH2B2 和 PLCG1，它们控制不同的信号级联。GRB2-Ras-MAPK 级联受 SHC1、FRS2、SH2B1 和 SH2B2 调节，参与神经元分化和神经突生长。Ras-PI3 激酶-AKT1 级联也由这些效应器控制，主要调节细胞生长和存活。TrkB 还在突触可塑性中发挥作用，并参与学习和记忆。通过 PLCG1，TrkB 激活 NF-Kappa-B 并促进与细胞存活相关的基因的转录。此外，TrkB 参与神经胶质细胞中中性粒细胞依赖性钙信号传导，并促进神经元和神经胶质细胞之间的通讯。总体而言，TrkB 在细胞存活和各种神经过程中发挥着至关重要的作用。

Measured by its binding ability in a functional ELISA. Immobilized Human BDNF at 1 μg/mL (100 μL/well) can bind Human TrkB Protein. The ED₅₀ for this effect is 17.97 ng/mL.

Human

HEK293

C-6*His

Q16620-1 (C32-H430)

4915  [NCBI]

CPTSCKCSASRIWCSDPSPGIVAFPRLEPNSVDPENITEIFIANQKRLEIINEDDVEAYVGLRNLTIVDSGLKFVAHKAFLKNSNLQHINFTRNKLTSLSRKHFRHLDLSELILVGNPFTCSCDIMWIKTLQEAKSSPDTQDLYCLNESSKNIPLANLQIPNCGLPSANLAAPNLTVEEGKSITLSCSVAGDPVPNMYWDVGNLVSKHMNETSHTQGSLRITNISSDDSGKQISCVAENLVGEDQDSVNLTVHFAPTITFLESPTSDHHWCIPFTVKGNPKPALQWFYNGAILNESKYICTKIHVTNHTEYHGCLQLDNPTHMNNGDYTLIAKNEYGKDEKQISAHFMGWPGIDDGANPNYPDVIYEDYGTAANDIGDTTNRSNEIPSTDVTDKTGREH

Approximately 60-105 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.