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  3. TSPAN8 蛋白, Human (HEK293, Fc)

TSPAN8 蛋白, Human (HEK293, Fc)

目录号: HY-P76118
产品使用指南

TSPAN8一种与整合素复合的细胞表面糖蛋白,属于四跨蛋白家族成员,具有四个疏水结构域。TSPAN8介导细胞外信号转导过程,调节细胞的发育、激活、生长和运动,此外 TSPAN8 在不同的癌症中表达,并且在癌症预后和靶向治疗中有潜在作用。TSPAN8 蛋白, Human (HEK293, Fc) 是重组的 TSPAN8 蛋白,由 HEK293 表达,带有 N-mFc 标签。TSPAN8 蛋白, Human (HEK293, Fc) 全长 96 个氨基酸,分子量约为 ~46 kDa。

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描述

TSPAN8 is a cell surface glycoprotein that is complexed with integrins and belongs to the four-span protein family. It has four hydrophobic domains. TSPAN8 mediates extracellular signal transduction processes and regulates cell development, activation, growth, and movement. In addition, TSPAN8 is expressed in different cancers and has potential roles in cancer prognosis and targeted therapy. TSPAN8 Protein, Human (HEK293, Fc) is the recombinant human-derived TSPAN8 protein, expressed by HEK293 , with N-mFc labeled tag. The total length of TSPAN8 Protein, Human (HEK293, Fc) is 96 a.a., with molecular weight of ~46 kDa.

研究背景

TSPAN8 通过激活 EGFR/AKT 通路,在促进肿瘤转移中发挥重要作用[1]
TSPAN8 通过靶向 mTORC2 来缓解高糖诱导的 HK-2 细胞自噬和凋亡 (apoptosis)[2]
TSPAN8 通过与自身和其他各种细胞信号分子相互作用形成TSPAN8 介导的蛋白复合物。这些蛋白质复合物有助于构建富含四跨膜蛋白的微区 (TEMs),有效地介导细胞内信号转导。此外,TSPAN8 在调节白细胞迁移、血管生成和伤口修复等生物学功能中起着至关重要的作用[3]

种属

Human

表达系统

HEK293

标签

N-mFc

蛋白编号

P19075 (K110-N205)

基因 ID
蛋白结构
N-term
mFc
TSPAN8 (K110-N205)
Accession # P19075
C-term
同用名
Tetraspanin-8; Tspan-8; CO-029; TSPAN8; TM4SF3
分子量

Approximately 46 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

TSPAN8 Protein, Human (HEK293, Fc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
TSPAN8 Protein, Human (HEK293, Fc)
目录号:
HY-P76118
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