Tubulin cofactor A 蛋白, Human (Tag Free)

The tubulin cofactor A protein is a key tubulin folding protein that initiates the tubulin folding pathway within the supercomplex (cofactors A to E). Cofactors A and D capture tubulin and stabilize it in a quasi-native state. Tubulin cofactor A Protein, Human (Tag Free) is the recombinant human-derived Tubulin cofactor A protein, expressed by E. coli , with tag free. The total length of Tubulin cofactor A Protein, Human (Tag Free) is 108 a.a., with molecular weight of ~14 kDa.

微管蛋白辅因子 A 蛋白作为微管蛋白折叠蛋白发挥着至关重要的作用，积极参与微管蛋白折叠途径的初始阶段。它是由辅因子 A 至 E 组成的超级复合物的一部分。辅因子 A 和 D 通过捕获和稳定微管蛋白的准天然构象发挥着关键作用。辅因子 E 与辅因子 D-微管蛋白复合物的相互作用促进随后与辅因子 C 的结合，导致微管蛋白多肽的释放，致力于采用天然状态。在协调这些复杂的步骤时，微管蛋白辅因子 A 蛋白在微管蛋白折叠的早期阶段发挥着关键作用，有助于形成功能性且稳定的微管蛋白结构。

Human

E. coli

Tag Free

O75347 (M1-A108)

6902  [NCBI]

MADPRVRQIKIKTGVVKRLVKEKVMYEKEAKQQEEKIEKMRAEDGENYDIKKQAEILQESRMMIPDCQRRLEAAYLDLQRILENEKDLEEAEEYKEARLVLDSVKLEA

Approximately 14 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.