TWEAK/TNFSF12 蛋白, Human (HEK293, hFc)

TWEAK/TNFSF12 Protein binds to FN14 and potentially TNRFSF12/APO3, weakly inducing apoptosis in certain cells. It activates NF-kappa-B, enhances angiogenesis, and stimulates endothelial cell proliferation. Furthermore, TWEAK/TNFSF12 is implicated in inflammatory cytokine induction and promotes IL8 secretion. It forms a homotrimer and interacts with the angiogenic factor AGGF1/VG5Q. TWEAK/TNFSF12 Protein, Human (HEK293, hFc) is the recombinant human-derived TWEAK/TNFSF12 protein, expressed by HEK293, with C-hFc labeled tag. The total length of TWEAK/TNFSF12 Protein, Human (HEK293, hFc) is 207 a.a., with molecular weight of 50-60 kDa.

TWEAK/TNFSF12 蛋白对 FN14 和潜在的 TNRFSF12/APO3 表现出结合亲和力，在某些细胞类型中充当细胞凋亡的弱诱导剂。此外，TWEAK/TNFSF12 参与 NF-kappa-B 激活，从而介导多种细胞反应，包括血管生成和内皮细胞增殖。其作用延伸至诱导炎症细胞因子和促进 IL8 分泌。作为同源三聚体，TWEAK/TNFSF12 可能参与复杂的分子相互作用以协调其不同的功能。此外，它与血管生成因子 AGGF1/VG5Q 相互作用，进一步强调了它参与血管生成及其作为细胞过程调节剂的潜力，对炎症和血管稳态具有影响。

Immobilized Human TNFSF12, hFc Tag at 5 μg/mL (100 μl/well) on the plate. Dose response curve for Biotinylated Anti-TNFSF12 Antibody, hFc Tag with the EC₅₀ of ≤0.11 μg/mL determined by ELISA.

Human

HEK293

C-hFc

O43508-1 (S43-H249)

8742  [NCBI]

50-60 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.