TYRO3/DTK 蛋白, Human (Q261H, HEK293, Fc)

The TYRO3/DTK protein is a tyrosine kinase receptor. TYRO3 transduces signals from the extracellular matrix by activating the PI3K/AKT/ NF-kappa-B pathway. TYRO3 plays an important role in inhibiting Toll-like receptor (TLRs) mediated innate immune response by activating STAT1. TYRO3 has the function of promoting tumor cell survival and/or proliferation, metastasis and chemotherapy resistance. TYRO3/DTK Protein, Human (Q261H, HEK293, Fc) is the recombinant human-derived TYRO3/DTK protein, expressed by HEK293 , with C-hFc labeled tag. The total length of TYRO3/DTK Protein, Human (Q261H, HEK293, Fc) is 388 a.a., with molecular weight of 85-120 kDa.

TYRO3/DTK 是一种受体酪氨酸激酶，通过与包括 TULP1 或 GAS6 在内的几种配体结合将信号从细胞外基质转导到细胞质。调节许多生理过程，包括细胞存活、迁移和分化。配体在细胞表面的结合诱导了 TYRO3 在胞内区域的二聚化和自磷酸化，为下游信号分子提供了对接位点。在配体激活后，与 PIK3R1 相互作用，从而增强 PI3 激酶活性。激活 AKT 存活通路，包括 NF-kappa-B 的核易位和 NF-kappa-B 调控基因的转录上调。TYRO3 信号在多种过程中发挥作用，如神经元保护免受兴奋性毒性损伤，血小板聚集和细胞骨架重组。通过激活 STAT1，在 Toll 样受体 (TLRs) 介导的先天免疫应答中发挥重要作用，STAT1 选择性地诱导细胞因子信号通路 SOCS1 和 SOCS3 的抑制因子的产生。TYRO3 在许多类型的癌症中过表达，并具有促进肿瘤细胞存活和/或增殖、转移和化疗耐药的功能。

Immobilized TYRO3 at 2 μg/mL (100 μL/well) can bind Biotinylated GAS6 protein. The ED₅₀ for this effect is 161.5 ng/mL, corresponding to a specific activity is 6.19×10^3 Unit/mg.

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  Immobilized TYRO3 at 2 μg/mL (100 μL/well) can bind Biotinylated GAS6 protein. The ED₅₀ for this effect is 161.5 ng/mL, corresponding to a specific activity is 6.19×10³ Unit/mg.

Human

HEK293

C-hFc

Q06418/AAH51756.1 (A41-S428, Q261H)

7301  [NCBI]

AGLKLMGAPVKLTVSQGQPVKLNCSVEGMEEPDIQWVKDGAVVQNLDQLYIPVSEQHWIGFLSLKSVERSDAGRYWCQVEDGGETEISQPVWLTVEGVPFFTVEPKDLAVPPNAPFQLSCEAVGPPEPVTIVWWRGTTKIGGPAPSPSVLNVTGVTQSTMFSCEAHNLKGLASSRTATVHLQALPAAPFNITVTKLSSSNASVAWMPGADGRALLQSCTVHVTQAPGGWEVLAVVVPVPPFTCLLRDLVPATNYSLRVRCANALGPSPYADWVPFQTKGLAPASAPQNLHAIRTDSGLILEWEEVIPEAPLEGPLGPYKLSWVQDNGTQDELTVEGTRANLTGWDPQKDLIVRVCVSNAVGCGPWSQPLVVSSHDRAGQQGPPHSRTS

85-120 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized a 0.22 μm filtered solution of 50 mM Tris-HCL, 150 mM NaCL, 100 mM arginine, 100 mM Glycine, pH 7.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.