1. 重组蛋白
  2. Enzymes & Regulators
  3. Oxidoreductases (EC 1)
  4. Tyrosine Hydroxylase 蛋白, Mouse (sf9, His)

Tyrosine Hydroxylase 蛋白, Mouse (sf9, His)

目录号: HY-P73563
COA 产品使用指南

酪氨酸羟化酶蛋白是儿茶酚胺(包括多巴胺、去甲肾上腺素和肾上腺素)生物合成的关键催化剂,并监督 L-酪氨酸向 L-二羟基苯丙氨酸 (L-Dopa) 的转化,这是合成中的关键和限速步骤途径。合成。Tyrosine Hydroxylase 蛋白, Mouse (sf9, His) 是重组的 酪氨酸羟化酶蛋白,由 Sf9 insect cells 表达,带有 N-His 标签。

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规格 价格 是否有货 数量
10 μg ¥1250 In-stock
20 μg ¥2000 询价
50 μg ¥3850 In-stock
100 μg ¥6200 询价
> 100 μg   询价  

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Tyrosine Hydroxylase Protein, Mouse (sf9, His) 相关产品:

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

Tyrosine hydroxylase protein is a key catalyst in the biosynthesis of catecholamines, including dopamine, norepinephrine, and epinephrine, and oversees the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa) , which is the critical and rate-limiting step pathway in synthesis.Tyrosine Hydroxylase Protein, Mouse (sf9, His) is the recombinant mouse-derived Tyrosine Hydroxylase protein, expressed by Sf9 insect cells , with N-His labeled tag.

研究背景

酪氨酸羟化酶是一种在儿茶酚胺(包括多巴胺、去甲肾上腺素和肾上腺素)的生物合成中发挥关键作用的酶。它催化该途径中的限速步骤,通过使用四氢生物蝶呤和分子氧将 L-酪氨酸转化为 L-二羟基苯丙氨酸 (L-多巴)。虽然其主要底物是酪氨酸,但酪氨酸羟化酶也表现出较低的特异性,并且可以羟基化苯丙氨酸和色氨酸。这种酶活性对于参与各种生理过程的神经递质的合成至关重要。此外,酪氨酸羟化酶被发现在出生后发育过程中积极调节视网膜玻璃体血管的退化,表明其除了儿茶酚胺生物合成之外还具有多种作用。

种属

Mouse

表达系统

Sf9 insect cells

标签

N-His

蛋白编号

P24529 (P2-S498)

基因 ID
蛋白结构
N-term
His
TyrH (P2-S498)
Accession # P24529
C-term
同用名
DYT14; TH; TYH dystonia 14; TYH; Tyrosine 3-monooxygenase
分子量

Approximately 65 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

溶液

组分

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.5, 10% Glycerol.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

Tyrosine Hydroxylase Protein, Mouse (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Tyrosine Hydroxylase Protein, Mouse (sf9, His)
目录号:
HY-P73563
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