1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. UB2D3 蛋白, Human (Sf9, His, Strep)

UB2D3 蛋白, Human (Sf9, His, Strep)

目录号: HY-P702065
产品使用指南

UBE2D3 蛋白是泛素化的关键参与者。它接受 E1 复合物中的泛素,并将其共价连接到多种蛋白质上,展现出多功能性。它在体外催化“Lys-11”和“Lys-48”连接的多聚泛素化,调节蛋白质泛素化模式。UB2D3 蛋白, Human (Sf9, His, Strep) 是重组的 UB2D3 蛋白,由 Sf9 insect cells 表达,带有 N-Strep, N-8*His 标签。UB2D3 蛋白, Human (Sf9, His, Strep) 全长 146 个氨基酸。

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描述

The UBE2D3 protein is a key player in ubiquitination. It accepts ubiquitin from the E1 complex and covalently attaches it to a variety of proteins, demonstrating multifunctionality. It catalyzes "Lys-11" and "Lys-48" linked polyubiquitination in vitro, regulating protein ubiquitination patterns. UB2D3 Protein, Human (Sf9, His, Strep) is the recombinant human-derived UB2D3 protein, expressed by Sf9 insect cells , with N-Strep, N-8*His labeled tag. The total length of UB2D3 Protein, Human (Sf9, His, Strep) is 146 a.a., .

研究背景

UBE2D3 是泛素化过程的关键参与者,它接受来自 E1 复合物的泛素,并协调其与多种蛋白质的共价连接,展示了其多功能性。在体外,UBE2D3 催化“Lys-11”和“Lys-48”连接的多泛素化,强调了其在调节蛋白质泛素化模式中的作用。UBE2D3 与 E2 CDC34 和 SCF(FBXW11) E3 连接酶复合物合作,有助于 NFKBIA 的多泛素化,从而导致随后的蛋白酶体降解。作为引发剂 E2,UBE2D3 在“Lys-21”和/或“Lys-22”位点用单泛素引发磷酸化 NFKBIA 靶标,这是 CDC34 泛素链延长之前的关键步骤。值得注意的是,UBE2D3 与 RNF8 一起充当启动子 E2,用于启动 PCNA,PCNA 在 S 期由紫外线或化学试剂引起的 DNA 损伤后激活的 DNA 损伤耐受途径中至关重要。此外,UBE2D3 与各种 E3 连接酶结合,包括 BRCA1/BARD1、MDM2、TOPORS、CBL、STUB1、RNF135 和 ZNF598,显示其参与多种泛素化过程,从 DNA 修复到病毒 RNA 依赖性信号通路的调节。

种属

Human

表达系统

Sf9 insect cells

标签

N-Strep;N-8*His

蛋白编号

P61077 (A2-M147)

基因 ID

/

蛋白结构
N-term
8*His-Strep
UB2D3 (A2-M147)
Accession # P61077
C-term
同用名
UBE2D3; Ubiquitin-conjugating enzyme E2 D3; (E3-independent) E2 ubiquitin-conjugating enzyme D3; E2 ubiquitin-conjugating enzyme D3; Ubiquitin carrier protein D3; Ubiquitin-conjugating enzyme E2(17)KB 3; Ubiquitin-conjugating enzyme E2-17 kDa 3; Ubiquitin-protein ligase D3
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

UB2D3 Protein, Human (Sf9, His, Strep) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
UB2D3 Protein, Human (Sf9, His, Strep)
目录号:
HY-P702065
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