UBA5 蛋白, Human (His)

The UBA5 protein activates UFM1 in ufmylation, linking the C-terminal glycine residue of UFM1 to a cysteine residue in E1. UBA5 Protein, Human (His) is the recombinant human-derived UBA5 protein, expressed by E. coli , with N-6*His labeled tag.

UBA5 作为一种 E1 样酶，在 ufmylation（一种翻译后修饰过程）的启动中发挥着关键作用。UFM1 的激活始于 UBA5 与 ATP 结合对其 C 末端甘氨酸残基进行腺苷酸化。这导致 UFM1-E1 硫酯和游离 AMP 的形成。值得注意的是，UBA5 通过反式结合机制实现了这一点，其中 UFM1 与 UBA5 同二聚体的两个亚基中的不同位点相互作用。这种反式结合不仅有利于 UBA5 同二聚体的稳定，而且还增强了 ATP 结合。随后，同样采用反式机制，UFM1 从 UBA5 转移到类 E2 酶 UFC1。由 UBA5 精心策划的 Ufmylation 是内质网应激引发的网状自噬 (ER-phagy) 中的一个关键过程。此外，Ufmylation 被证明对于巨核细胞和红细胞的红细胞分化是必不可少的。UBA5 以同型二聚体形式存在，这种同型二聚体对于 UFM1 激活至关重要。UBA5 还与 UFM1、GABARAPL2、GABARAP、GABARAPL1 和 UFC1 发生特定的相互作用，强调了它在多种细胞过程中的复杂参与。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His

Q9GZZ9-1 (M1-M404)

79876  [NCBI]

MAESVERLQQRVQELERELAQERSLQVPRSGDGGGGRVRIEKMSSEVVDSNPYSRLMALKRMGIVSDYEKIRTFAVAIVGVGGVGSVTAEMLTRCGIGKLLLFDYDKVELANMNRLFFQPHQAGLSKVQAAEHTLRNINPDVLFEVHNYNITTVENFQHFMDRISNGGLEEGKPVDLVLSCVDNFEARMTINTACNELGQTWMESGVSENAVSGHIQLIIPGESACFACAPPLVVAANIDEKTLKREGVCAASLPTTMGVVAGILVQNVLKFLLNFGTVSFYLGYNAMQDFFPTMSMKPNPQCDDRNCRKQQEEYKKKVAALPKQEVIQEEEEIIHEDNEWGIELVSEVSEEELKNFSGPVPDLPEGITVAYTIPKKQEDSVTELTVEDSGESLEDLMAKMKNM

14&40-60 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, 10% Glycerol, pH 8.0 .

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.