1. 重组蛋白
  2. Ubiquitin Related Proteins
  3. Ubiquitin Enzymes
  4. E2 Enzymes
  5. Ubiquitin Conjugating Enzyme E2 R2
  6. UBE2R2 蛋白, Human (His)

UBE2R2 蛋白是泛素蛋白酶体系统的关键元件,具有 E2 泛素结合酶的功能。它接受 E1 复合物中的泛素并催化其与多种蛋白质底物的共价附着。UBE2R2 蛋白, Human (His) 是重组的 UBE2R2 蛋白,由 E. coli 表达,带有 N-6*His 标签。UBE2R2 蛋白, Human (His) 全长 238 个氨基酸,分子量约为 ~37.0 kDa。

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描述

The UBE2R2 protein is a key element of the ubiquitin-proteasome system and functions as an E2 ubiquitin-conjugating enzyme. It accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to a variety of protein substrates. UBE2R2 Protein, Human (His) is the recombinant human-derived UBE2R2 protein, expressed by E. coli , with N-6*His labeled tag. The total length of UBE2R2 Protein, Human (His) is 238 a.a., with molecular weight of ~37.0 kDa.

研究背景

UBE2R2 是泛素-蛋白酶体系统的一个组成部分,通过接受 E1 复合物中的泛素并催化其与不同蛋白质底物的共价附着,起到 E2 泛素结合酶的作用。在体外测定中,UBE2R2 表现出多种催化活性,促进单泛素化和“Lys-48”连接的多泛素化反应。这表明它可能参与各种细胞过程,包括蛋白质的靶向降解,这可能包括链蛋白或其他相关底物的调节。

种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

Q712K3 (M1-S238)

基因 ID
蛋白结构
N-term
6*His
UBE2R2 (M1-S238)
Accession # Q712K3
C-term
中文名
UBE2R2 蛋白
同用名
Ubiquitin-Conjugating Enzyme E2 R2; Ubiquitin Carrier Protein R2; Ubiquitin-Conjugating Enzyme E2-CDC34B; Ubiquitin-Protein Ligase R2; UBE2R2; CDC34B; UBC3B
氨基酸序列

MAQQQMTSSQKALMLELKSLQEEPVEGFRITLVDESDLYNWEVAIFGPPNTLYEGGYFKAHIKFPIDYPYSPPTFRFLTKMWHPNIYENGDVCISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMFRKWRDSKGKDKEYAEIIRKQVSATKAEAEKDGVKVPTTLAEYCIKTKVPSNDNSSDLLYDDLYDDDIDDEDEEEEDADCYDDDDSGNEES

分子量

Approximately 37.0 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 重组蛋白稀释计算器

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
UBE2R2 Protein, Human (His)
目录号:
HY-P71407
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