1. 重组蛋白
  2. Ubiquitin Related Proteins
  3. Ubiquitin Enzymes
  4. E2 Enzymes
  5. Ubiquitin Conjugating Enzyme E2 V2
  6. UBE2V2 蛋白, Human (His)

UBE2V2 蛋白, Human (His)

目录号: HY-P71411
产品使用指南

UBE2V2蛋白缺乏独立的泛素连接酶活性,并与UBE2N形成功能性异二聚体。它们共同催化非经典多聚泛素链合成(“Lys-63”),与蛋白酶体驱动的降解不同。UBE2V2 蛋白, Human (His) 是重组的 UBE2V2 蛋白,由 E. coli 表达,带有 N-6*His 标签。UBE2V2 蛋白, Human (His) 全长 145 个氨基酸,分子量约为 ~18.0 kDa。

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描述

UBE2V2 protein lacks independent ubiquitin ligase activity and forms a functional heterodimer with UBE2N. Together, they catalyze nonclassical polyubiquitin chain synthesis ("Lys-63"), distinct from proteasome-driven degradation. UBE2V2 Protein, Human (His) is the recombinant human-derived UBE2V2 protein, expressed by E. coli , with N-6*His labeled tag. The total length of UBE2V2 Protein, Human (His) is 145 a.a., with molecular weight of ~18.0 kDa.

研究背景

UBE2V2 蛋白单独作用时缺乏泛素连接酶活性,但与 UBE2N 形成功能性异二聚体,催化通过“Lys-63”连接的非规范多泛素链的合成。值得注意的是,这种类型的多聚泛素化不会导致蛋白酶体降解蛋白质。UBE2V2 在介导靶基因的转录激活、影响细胞周期进程以及促进细胞分化方面发挥着关键作用。此外,它还参与无错误的 DNA 修复途径,增强 DNA 损伤后的细胞存活率。UBE2V2 主要作为 UBE2N 的异二聚体发挥作用,并表现出与 CHFR 的结合亲和力,表明其参与各种细胞过程和分子相互作用。

种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

Q15819 (M1-N145)

基因 ID
蛋白结构
N-term
6*His
UBE2V2 (M1-N145)
Accession # Q15819
C-term
同用名
Ubiquitin-Conjugating Enzyme E2 Variant 2; DDVit 1; Enterocyte Differentiation-Associated Factor 1; EDAF-1; Enterocyte Differentiation-Promoting Factor 1; EDPF-1; MMS2 Homolog; Vitamin D3-Inducible Protein; UBE2V2; MMS2; UEV2
氨基酸序列

MAVSTGVKVPRNFRLLEELEEGQKGVGDGTVSWGLEDDEDMTLTRWTGMIIGPPRTNYENRIYSLKVECGPKYPEAPPSVRFVTKINMNGINNSSGMVDARSIPVLAKWQNSYSIKVVLQELRRLMMSKENMKLPQPPEGQTYNN

分子量

Approximately 18.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
UBE2V2 Protein, Human (His)
目录号:
HY-P71411
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