UCHL1 蛋白, Rat (His)

The UCHL1 protein is a ubiquitin proteolytic enzyme essential for processing ubiquitin precursors and hydrolyzing ubiquitinated proteins. As a thiol protease, it specifically cleaves the C-terminal glycine of ubiquitin. UCHL1 Protein, Rat (His) is the recombinant rat-derived UCHL1 protein, expressed by E. coli , with N-10*His labeled tag. The total length of UCHL1 Protein, Rat (His) is 222 a.a., with molecular weight of ~28 kDa.

UCHL1 蛋白是一种泛素蛋白水解酶，在泛素前体和泛素化蛋白的加工中发挥着至关重要的作用。作为硫醇蛋白酶，它特异性识别并水解泛素 C 末端甘氨酸的肽键。此外，UCHL1 蛋白具有与游离单泛素结合的能力，可能防止其在溶酶体中降解。此外，UCHL1 蛋白的同二聚体形式可能表现出不依赖于 ATP 的泛素连接酶活性。

Rat

E. coli

N-10*His

Q00981 (Q2-A223)

29545  [NCBI]

QLKPMEINPEMLNKVLAKLGVAGQWRFADVLGLEEETLGSVPSPACALLLLFPLTAQHENFRKKQIEELKGQEVSPKVYFMKQTIGNSCGTIGLIHAVANNQDKLEFEDGSVLKQFLSETEKLSPEDRAKCFEKNEAIQAAHDSVAQEGQCRVDDKVNFHFILFNNVDGHLYELDGRMPFPVNHGASSEDSLLQDAAKVCREFTEREQGEVRFSAVALCKAA

Approximately 28 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.