1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. UMP-CMP kinase/CMPK1 蛋白, Human (sf9, His)

UMP-CMP kinase/CMPK1 蛋白, Human (sf9, His)

目录号: HY-P77273A
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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

生物活性

Measured by its ability to catalyze 1mM substrate CMP and 50 μM ATP that incubate at 37°C for 30 minutes. The specific activity is 52.56 pmo/min/μg.

种属

Human

表达系统

Sf9 insect cells

标签

N-6*His

蛋白编号

P30085-1 (M1-G196)

基因 ID

51727

同用名
Deoxycytidylate kinase; CK; CMK; CMPK; UMK; UMPK
氨基酸序列

MKPLVVFVLGGPGAGKGTQCARIVEKYGYTHLSAGELLRDERKNPDSQYGELIEKYIKEGKIVPVEITISLLKREMDQTMAANAQKNKFLIDGFPRNQDNLQGWNKTMDGKADVSFVLFFDCNNEICIERCLERGKSSGRSDDNRESLEKRIQTYLQSTKPIIDLYEEMGKVKKIDASKSVDEVFDEVVQIFDKEG

分子量

approximately 24 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCL, 500 mM NaCl, pH 7.4, 10% Glycerol.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

UMP-CMP kinase/CMPK1 Protein, Human (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
UMP-CMP kinase/CMPK1 Protein, Human (sf9, His)
目录号:
HY-P77273A
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