UQCRH 蛋白, Human (GST)

UQCRH is an important component of the mitochondrial electron transport chain and contributes to oxidative phosphorylation within the ubiquinol-cytochrome c oxidoreductase complex. It operates in the respiratory chain, transferring electrons from NADH and succinate to molecular oxygen, establishing an electrochemical gradient for ATP synthesis. UQCRH Protein, Human (GST) is the recombinant human-derived UQCRH protein, expressed by E. coli , with N-GST labeled tag. The total length of UQCRH Protein, Human (GST) is 91 a.a., with molecular weight of 33-45 kDa.

UQCRH 是泛醇细胞色素 c 氧化还原酶的重要组成部分，是氧化磷酸化所必需的线粒体电子传递链的关键组成部分。作为呼吸链（包括琥珀酸脱氢酶（复合物 II）、泛醇-细胞色素 c 氧化还原酶（复合物 III）和细胞色素 c 氧化酶（复合物 IV））的一部分，UQCRH 协作将电子从 NADH 和琥珀酸转移到分子氧，从而建立了呼吸链跨内膜的电化学梯度驱动 ATP 合成酶和跨膜运输。UQCRH 在细胞色素 b-c1 复合物内运作，催化电子从泛醇到细胞色素 c 的转移，将这种氧化还原反应与质子穿过线粒体内膜的易位耦合起来。在 Q 循环过程中，质子从基质中被消耗，释放到膜间空间，电子被传递到细胞色素 c。UQCRH 是多亚基泛醇-细胞色素 c 氧化还原酶复合物的组成部分，由各种亚基组成，包括呼吸蛋白和核心蛋白，与 NADH-泛醌氧化还原酶（复合物 I）和细胞色素等其他呼吸复合物在线粒体内膜中形成必需二聚体和超复合物c氧化酶（复合体IV）。

Human

E. coli

N-GST

P07919 (M1-K91)

7388  [NCBI]

MGLEDEQKMLTESGDPEEEEEEEEELVDPLTTVREQCEQLEKCVKARERLELCDERVSSRSHTEEDCTEELFDFLHARDHCVAHKLFNNLK

33-45 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.