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  3. UROIIIS 蛋白, Human (His)

UROIIIS 蛋白, Human (His)

目录号: HY-P71105
产品使用指南

UROIIIS 蛋白是生化过程中的关键催化剂,特别是催化羟甲基双烷(一种线性四吡咯)环化形成大环尿卟啉原 III。该酶促步骤是通往负责合成不同卟啉的各种子途径的关键分支点。UROIIIS 蛋白, Human (His) 是重组的 UROIIIS 蛋白,由 E. coli 表达,带有 C-6*His 标签。UROIIIS 蛋白, Human (His) 全长 265 个氨基酸,分子量约为 ~29.0 kDa。

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描述

The UROIIIS protein is a key catalyst in biochemical processes, specifically catalyzing the cyclization of hydroxymethyl bixane, a linear tetrapyrrole, to form macrocyclic uroporphyrinogen III. This enzymatic step serves as a critical branching point leading to various subpathways responsible for the synthesis of different porphyrins. UROIIIS Protein, Human (His) is the recombinant human-derived UROIIIS protein, expressed by E. coli , with C-6*His labeled tag. The total length of UROIIIS Protein, Human (His) is 265 a.a., with molecular weight of ~29.0 kDa.

研究背景

UROIIIS 或尿卟啉原 III 合酶通过催化羟甲基联烷环化形成大环尿卟啉原 III,在四吡咯生物合成中发挥关键作用。这一酶促步骤代表了一个关键的分支点,产生了各种子途径,从而产生了多种卟啉。通过这一过程合成的卟啉是参与不同细胞过程的多种酶的必需辅助因子。这些过程包括甲硫氨酸合成(其中卟啉充当与维生素 B12 代谢相关的酶的辅助因子)以及氧运输(其中卟啉,特别是血红素,在血红蛋白中结合和运输氧气方面发挥着至关重要的作用)。参考文献强调了 UROIIIS 在卟啉生物合成中的核心作用及其通过生成必需辅因子支持各种生物过程的重要性。

种属

Human

表达系统

E. coli

标签

C-6*His

蛋白编号

P10746 (M1-C265)

基因 ID
蛋白结构
N-term
UROIIIS (M1-C265)
Accession # P10746
6*His
C-term
同用名
Uroporphyrinogen-III Synthase; UROIIIS; UROS; Hydroxymethylbilane Hydrolyase [Cyclizing]; Uroporphyrinogen-III Cosynthase; UROS
氨基酸序列

MKVLLLKDAKEDDCGQDPYIRELGLYGLEATLIPVLSFEFLSLPSFSEKLSHPEDYGGLIFTSPRAVEAAELCLEQNNKTEVWERSLKEKWNAKSVYVVGNATASLVSKIGLDTEGETCGNAEKLAEYICSRESSALPLLFPCGNLKREILPKALKDKGIAMESITVYQTVAHPGIQGNLNSYYSQQGVPASITFFSPSGLTYSLKHIQELSGDNIDQIKFAAIGPTTARALAAQGLPVSCTAESPTPQALATGIRKALQPHGCC

分子量

Approximately 29.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

UROIIIS Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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