USP10 蛋白, Human (Sf9, His, GST)

USP10 is a multifunctional hydrolase that plays a critical regulatory role in a variety of cellular processes. It stabilizes the tumor suppressor p53/TP53 by deubiquitinating the tumor suppressor p53/TP53 in the cytoplasm, thereby counteracting MDM2-mediated degradation. USP10 Protein, Human (Sf9, His, GST) is the recombinant human-derived USP10, expressed by Sf9 insect cells , with His, GST labeled tag. ,

USP10 是一种具有多种底物特异性的水解酶，是多种细胞过程中的关键调节剂。值得注意的是，它在无应激条件下在细胞质中特异性去泛素化肿瘤抑制因子 p53/TP53，从而抵消 MDM2 介导的降解，从而在稳定肿瘤抑制因子 p53/TP53 方面发挥着关键作用。DNA 损伤后，USP10 易位至细胞核，继续使 p53/TP53 去泛素化，从而有助于调节 DNA 损伤反应。此外，USP10 是控制自噬和 p53/TP53 水平的调节环路不可或缺的一部分，介导 BECN1 的去泛素化。在核糖体生物学中，USP10 参与 40S 核糖体亚基回收，抑制应激颗粒形成并防止 40S 核糖体蛋白在翻译停滞期间降解。除了在蛋白质稳定性方面的作用外，USP10 通过使早期内体中的 CFTR 去泛素化来参与内吞再循环，并参与 TANK 依赖性负反馈反应，以减弱 IL1B 刺激或 DNA 损伤时的 NF-kappa-B 激活。此外，USP10 通过去泛素化稳定 TBX21，表明其参与多种细胞途径。

The fundamental role of USP10 is specific removal of ubiquitin from substrates. USP10 catalyses the ubiquitin from the substrate Ub-Rho110 to release fluorophores. Rho110 will release 535nM emission light under the excitation condition of 485nM.

Human

Sf9 insect cells

His;GST

Q14694 (A2-L798)

9100

Approximately 114.7 kDa

Greater than 85% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 200 mM NaCl, 20% glycerol, 1 mM DTT, pH 7.5.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.