USP11 蛋白, Human (Sf9)

The USP11 protein is a multifunctional protease that selectively removes ubiquitin from target proteins and polyubiquitin chains, thereby hindering their proteasomal degradation. It shows a preference for “Lys-6” and “Lys-63” linked ubiquitin chains and less activity towards other linkages. USP11 Protein, Human (Sf9) is the recombinant human-derived USP11 protein, expressed by Sf9 insect cells , with tag free. The total length of USP11 Protein, Human (Sf9) is 962 a.a., .

USP11 蛋白作为蛋白酶发挥作用，能够从靶蛋白和多聚泛素链上去除缀合的泛素，从而抑制蛋白酶体对其的降解。USP11 表现出对“Lys-6”和“Lys-63”连接的泛素链的偏好，对“Lys-11”和“Lys-33”连接的链表现出较低的活性，对“Lys-27”的活性最低，体外“Lys-29”和“Lys-48”连接链。除了其蛋白水解作用之外，USP11 也是导致 NF-kappa-B 激活的途径调节中不可或缺的一部分，并且在双链 DNA 断裂后的 DNA 修复中发挥着至关重要的作用。此外，它还通过与 Polycomb 组 (PcG) 多蛋白 PRC1 样复合物结合，发挥染色质调节剂的作用，可能是通过使该复合物的成分去泛素化而发挥作用。USP11 参与细胞增殖的特点在于其使磷酸化 E2F1 去泛素化的能力，进一步强调了其在细胞过程中的多方面作用。

The fundamental role of USP11 is specific removal of ubiquitin from substrates. USP11 catalyses the ubiquitin from the substrate Ub-Rho110 to release fluorophores. Rho110 will release 535 nM emission light under the excitation condition of 485 nM. The signal of which can be quickly and reliably captured using a microplate reader.

Human

Sf9 insect cells

Tag Free

P51784 (A2-N963)

/

Approximately 109.8 kDa

Greater than 85% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH7.5, 200 mM NaCl, 20% glycerol.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.