USP2 蛋白, Human

USP2 catalytic domain proteins act as hydrolases and target polyubiquitinated proteins such as MDM2, MDM4, and CCND1. Both isoform 1 and isoform 4 exhibit ubiquitin-specific peptidase and isopeptidase activities. USP2 Protein, Human is the recombinant human-derived USP2 protein, expressed by E. coli , with tag free. The total length of USP2 Protein, Human is 347 a.a., .

USP2 催化结构域蛋白充当水解酶，靶向多泛素化蛋白，例如 MDM2、MDM4 和 CCND1。异构体 1 和异构体 4 均表现出泛素特异性肽酶和异肽酶活性。值得注意的是，它使 MDM2 去泛素化，间接促进 p53/TP53 降解并限制 p53 活性，而不逆转 MDM2 介导的 p53 泛素化。此外，USP2 可防止 MDM2 介导的 MDM4 降解，从而有助于细胞过程的复杂调节。在细胞周期进展的背景下，USP2 在正常细胞和癌细胞的 G1/S 转变中发挥作用。此外，它作为生物钟的调节器出现，影响其内在节律和对外部线索的反应。通过与时钟蛋白结合，USP2 使核心时钟组件 PER1 去泛素化，影响其核质穿梭和核保留。这种多面蛋白还参与胚胎肌肉细胞生肌分化的调节，并作为生物钟输出效应器，通过可能调节 NHERF4 水平来影响小肠中 Ca(2+) 的吸收，从而影响 Ca(2+) 的活性。2+) 通道 TRPV6。

The fundamental role of USP2 is specific removal of ubiquitin from substrates. USP2 catalyses the ubiquitin from the substrate Ub-Rho110 to release fluorophores. Rho110 will release 535 nM emission light under the excitation condition of 485 nM. The signal of which can be quickly and reliably captured using a microplate reader.

Human

E. coli

Tag Free

O75604 (N259-M605)

9099

Approximately 40 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, 500 mM NaCl, 5% glycerol, pH 8.0,

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.