1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. USP25 蛋白, Human (His)

USP25 蛋白, Human (His)

目录号: HY-P701445
产品使用指南

USP25 蛋白充当去泛素化酶,在与底物缀合的泛素部分的水解中发挥关键作用。该功能能够处理新合成的泛素、回收泛素分子以及编辑多聚泛素链,从而防止底物的蛋白酶体降解。USP25 蛋白, Human (His) 是重组的 USP25 蛋白,由 E. coli 表达,带有 N-6*His 标签。USP25 蛋白, Human (His) 全长 1054 个氨基酸。

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描述

The USP25 protein acts as a deubiquitinating enzyme and plays a key role in the hydrolysis of the ubiquitin moiety conjugated to the substrate. This function enables processing of newly synthesized ubiquitin, recycling of ubiquitin molecules, and editing of polyubiquitin chains, thus preventing proteasomal degradation of substrates. USP25 Protein, Human (His) is the recombinant human-derived USP25 protein, expressed by E. coli , with N-6*His labeled tag. The total length of USP25 Protein, Human (His) is 1054 a.a., .

研究背景

泛素特异性蛋白酶 25 (USP25) 是一种去泛素化酶,能够水解与底物缀合的泛素部分,从而参与新合成泛素的加工、回收泛素分子和编辑多泛素链。USP25 通过裂解泛素链在防止底物蛋白酶体降解方面发挥着至关重要的作用。值得注意的是,它对“Lys-48”和“Lys-63”连接的四泛素链均表现出水解活性,展示了其针对不同泛素连接类型的多功能性。此外,USP25 的肌肉特异性亚型(称为 USP25m)被认为在肌肉分化和功能的调节中发挥作用,表明其在特定细胞环境中的潜在重要性。

种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

Q9UHP3 (T2-R1055)

基因 ID

29761

蛋白结构
N-term
6*His
USP25 (T2-R1055)
Accession # Q9UHP3
C-term
同用名
USP25; Ubiquitin carboxyl-terminal hydrolase 25; Deubiquitinating enzyme 25; USP on chromosome 21; Ubiquitin thioesterase 25; Ubiquitin-specific-processing protease 25
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

USP25 Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
USP25 Protein, Human (His)
目录号:
HY-P701445
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