USP30 蛋白, Human (Sf9)

USP30 protein is anchored on the outer mitochondrial membrane and severely inhibits mitophagy by antagonizing Parkin (PRKN). Hydrolyzing ubiquitin on RHOT1/MIRO1 and target proteins such as TOMM20 and USP30 blocks Parkin-mediated mitophagy, thereby regulating the clearance of damaged mitochondria. USP30 Protein, Human (Sf9) is the recombinant human-derived USP30 protein, expressed by Sf9 insect cells , with tag free. The total length of USP30 Protein, Human (Sf9) is 461 a.a., .

USP30 蛋白锚定在线粒体外膜上，作为去泛素化酶发挥着关键作用，是线粒体自噬的关键抑制剂，对抗 Parkin (PRKN) 的作用。通过水解 Parkin 附着在 RHOT1/MIRO1 和 TOMM20 等靶蛋白上的泛素，USP30 阻碍 Parkin 驱动线粒体自噬的能力，从而调节受损线粒体的选择性清除。其底物特异性延伸至“Lys-6”和“Lys-11”连接的多聚泛素链的优先裂解，这是参与线粒体自噬信号传导的关键连接。值得注意的是，USP30 不能有效裂解“Ser-65”处磷酸化的多聚泛素。除了在线粒体自噬中的作用外，USP30 还充当线粒体融合的负调节因子，介导 MFN1 和 MFN2 的去泛素化。这种双重作用强调了 USP30 协调线粒体动力学和质量控制的复杂调节机制，在维持线粒体稳态中发挥着核心作用。

The fundamental role of USP30 is specific removal of ubiquitin from substrates. USP30 catalyses the ubiquitin from the substrate Ub-Rho110 to release fluorophores. Rho110 will release 535 nM emission light under the excitation condition of 485 nM. The signal of which can be quickly and reliably captured using a microplate reader.

Human

Sf9 insect cells

Tag Free

Q70CQ3 (T57-E517)

84749

Approximately 52.7 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.