USP9X 蛋白, Human (GST)

The USP9X protein serves as a deubiquitinase, playing a crucial role in processing ubiquitin precursors and preventing protein degradation. It exhibits specificity in hydrolyzing various polyubiquitin chain linkages, including "Lys-63", "Lys-48", "Lys-29" and "Lys-33". USP9X Protein, Human (GST) is the recombinant human-derived USP9X protein, expressed by E. coli , with N-GST labeled tag. The total length of USP9X Protein, Human (GST) is 442 a.a., .

根据本描述，USP9X 蛋白作为参与泛素前体和泛素化蛋白加工的去泛素酶发挥着至关重要的作用。USP9X 对蛋白质周转有显着影响，通过去除结合的泛素来防止降解。它在水解各种多聚泛素链连接方面表现出特异性，包括“Lys-63”、“Lys-48”、“Lys-29”和“Lys-33”。USP9X 是 TGF-β/BMP 信号级联的重要组成部分，可去泛素化 SMAD4 并对抗 E3 泛素蛋白连接酶 TRIM33 的活性。此外，它还与 OTUD4 合作，通过去泛素化 ALKBH3 来调节烷基化 DNA 损伤修复。USP9X 稳定 mTORC2 复合体成分 RICTOR，增强其与 MTOR 的结合并促进复合体组装。在有丝分裂中，它通过控制 BIRC5/survivin 在着丝粒上的定位来调节染色体排列和分离。USP9X 影响轴突生长、神经元细胞迁移和细胞时钟功能，增强 BMAL1 稳定性和转录活性。此外，它通过去泛素化 PEX5 和去泛素化 PEG10 来充当过氧化物酶体输入的调节剂。这种多方面的作用凸显了 USP9X 通过其去泛素化活性所协调的多种细胞功能。

Human

E. coli

N-GST

Q93008 (K1554-N1995)

8239

Greater than 80% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH7.5, 200 mM NaCl, 20% glycerol.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.