VAPB 蛋白, Human (His)

VAPB protein interacts with STARD3 through a phosphorylation-dependent mechanism to form a contact site between the endoplasmic reticulum (ER) and late endosomes. VAPB Protein, Human (His) is the recombinant human-derived VAPB protein, expressed by E. coli , with C-6*His labeled tag. The total length of VAPB Protein, Human (His) is 131 a.a., with molecular weight of ~17.0 kDa.

VAPB 蛋白是一种内质网锚定蛋白，通过以 FFAT 基序的磷酸化依赖性方式与 STARD3 相互作用，在内质网 (ER) 和晚期内体之间的接触位点形成中发挥着至关重要的作用。除了其结构参与之外，VAPB 通过诱导 ERN1/IRE1 活性来促进内质网未折叠蛋白反应 (UPR)。此外，它还参与细胞钙稳态的调节。VAPB 以同二聚体形式存在，并与 VAPA 形成异二聚体。其相互作用组延伸至包括 VAMP1、VAMP2、ZFYVE27、RMDN3、KIF5A、STARD3、STARD3NL、CERT1、PLEKHA3、SACM1L 和 VPS13A 等蛋白质，强调了其参与多种细胞过程的多功能性。与 RB1CC1、MIGA2、OSBPL1A、KCNB1 和 KCNB2 的相互作用（涉及磷酸化 FFAT 基序）凸显了 VAPB 参与的复杂调控网络。这种全面的相互作用网络强调了 VAPB 在协调 ER-内体界面的基本细胞过程中的关键作用。

Human

E. coli

C-6*His

O95292 (A2-P132)

9217  [NCBI]

AKVEQVLSLEPQHELKFRGPFTDVVTTNLKLGNPTDRNVCFKVKTTAPRRYCVRPNSGIIDAGASINVSVMLQPFDYDPNEKSKHKFMVQSMFAPTDTSDMEAVWKEAKPEDLMDSKLRCVFELPAENDKP

Approximately 17.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 100 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.