Vaspin 蛋白, Human

Vaspin Protein, Human, as a secretory adipokine, exerts a protective role on insulin resistance.

Vaspin 是一种有益的脂肪因子，可对抗肥胖小鼠的胰岛素抵抗和炎症过程。vaspin可通过AMPK-mTOR依赖性激活自噬流抑制心肌细胞凋亡，从而减轻心脏缺血/再灌注（I/R）损伤，从而为心肌I/R损伤和其他缺血性疾病的治疗提供潜在突破^[1][2]。此外，vaspin 可诱导 PI3K/AKT 轴的激活（与胰岛素受体激活无关），促进 GLUT4 表达和易位，并使老年肥胖人骨骼肌对胰岛素介导的葡萄糖摄取敏感^[3]。

Human

E. coli

Tag Free

Q8IW75 (L21-K414)

145264  [NCBI]

LKPSFSPRNYKALSEVQGWKQRMAAKELARQNMDLGFKLLKKLAFYNPGRNIFLSPLSISTAFSMLCLGAQDSTLDEIKQGFNFRKMPEKDLHEGFHYIIHELTQKTQDLKLSIGNTLFIDQRLQPQRKFLEDAKNFYSAETILTNFQNLEMAQKQINDFISQKTHGKINNLIENIDPGTVMLLANYIFFRARWKHEFDPNVTKEEDFFLEKNSSVKVPMMFRSGIYQVGYDDKLSCTILEIPYQKNITAIFILPDEGKLKHLEKGLQVDTFSRWKTLLSRRVVDVSVPRLHMTGTFDLKKTLSYIGVSKIFEEHGDLTKIAPHRSLKVGEAVHKAELKMDERGTEGAAGTGAQTLPMETPLVVKIDKPYLLLIYSEKIPSVLFLGKIVNPIGK

40-50 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Zieger K, et al. Vaspin suppresses cytokine-induced inflammation in 3T3-L1 adipocytes via inhibition of NFκB pathway. Mol Cell Endocrinol. 2018 Jan 15;460:181-188.  [Content Brief]

  [2]. Yang F, et al. Vaspin alleviates myocardial ischaemia/reperfusion injury via activating autophagic flux and restoring lysosomal function. Biochem Biophys Res Commun. 2018 Sep 5;503(2):501-507.  [Content Brief]