VCP 蛋白, Human (His)

VCP proteins are indispensable for Golgi stack dynamics, coordinating fragmentation during mitosis and postmitotic reorganization. It actively promotes the formation of the transitional endoplasmic reticulum (tER), enables ATP-dependent vesicle budding, and enables membrane transfer to the Golgi apparatus. VCP Protein, Human (His) is the recombinant human-derived VCP protein, expressed by E. coli , with C-6*His labeled tag. The total length of VCP Protein, Human (His) is 588 a.a., with molecular weight of ~75.0 kDa.

VCP 蛋白对于协调与高尔基体堆叠相关的动态过程至关重要，在有丝分裂期间的碎片以及随后的有丝分裂后的重组中发挥着至关重要的作用。它积极参与移行内质网 (tER) 的形成，通过从 tER 出芽的 ATP 依赖性囊泡，促进膜从内质网转移到高尔基体。VCP、UFD1 和 NPLOC4 三元复合物对于结合泛素化蛋白并介导错误折叠蛋白从内质网输出到细胞质以进行蛋白酶体降解至关重要。此外，VCP 是各种细胞过程不可或缺的一部分，包括纺锤体解体的调节、闭合核膜的形成以及 E3 泛素蛋白连接酶活性的调节。它积极参与内质网应激诱导的质量控制，并通过参与修复双链断裂和停滞的复制叉，在 DNA 损伤反应中发挥至关重要的作用。此外，VCP 参与清除过程，介导应激线粒体外膜蛋白的提取并促进含有泛素的自噬体的成熟。其多方面功能延伸至I型干扰素产生的负调节，在膜蛋白向溶酶体的泛素依赖性分选中发挥作用，并通过控制IGF1R的稳态表达来间接调节胰岛素样生长因子受体信号通路受体。

No Enzyme activity.

Human

E. coli

C-6*His

P55072 (A2-N589)

7415  [NCBI]

ASGADSKGDDLSTAILKQKNRPNRLIVDEAINEDNSVVSLSQPKMDELQLFRGDTVLLKGKKRREAVCIVLSDDTCSDEKIRMNRVVRNNLRVRLGDVISIQPCPDVKYGKRIHVLPIDDTVEGITGNLFEVYLKPYFLEAYRPIRKGDIFLVRGGMRAVEFKVVETDPSPYCIVAPDTVIHCEGEPIKREDEEESLNEVGYDDIGGCRKQLAQIKEMVELPLRHPALFKAIGVKPPRGILLYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPAIIFIDELDAIAPKREKTHGEVERRIVSQLLTLMDGLKQRAHVIVMAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEILQIHTKNMKLADDVDLEQVANETHGHVGADLAALCSEAALQAIRKKMDLIDLEDETIDAEVMNSLAVTMDDFRWALSQSNPSALRETVVEVPQVTWEDIGGLEDVKRELQELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKARQAAPCVLFFDELDSIAKARGGN

Approximately 75.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 50 mM Tris-HCl, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.