VEGF120 蛋白, Mouse

VEGF-A Protein is a key member of the VEGF family of cytokines.VEGF-A participates in angiogenesis, vasculogenesis, and endothelial cell growth, inducing endothelial cell proliferation, promoting cell migration, inhibiting cell apoptosis, and inducing vascular permeability.VEGF-A stimulates endothelial cell mitogenesis and cell migration.VEGF120 Protein, Mouse is the recombinant mouse-derived VEGF120 protein, expressed by E.coli , with tag free.

VEGF-A 是 VEGF 细胞因子家族的重要成员，其他成员还有 VEGF-B、-C、-D 和 PGF。VEGF-A 参与血管生成、血管发生和内皮细胞生长，诱导内皮细胞增殖、促进细胞迁移、抑制细胞凋亡、诱导血管通透性。VEGF-A 与 FLT1/VEGFR1、KDR/VEGFR2 和 DEAR/FBXW7-AS1 受体、硫酸乙酰肝素和肝素结合。VEGF-A 还与 NRP1 结合，启动运动神经元轴突引导和细胞体迁移所需的信号通路，包括胚胎发育过程中面部运动神经元从菱形 4 到菱形 6 的尾部迁移。VEGF-A 刺激内皮细胞有丝分裂和细胞迁移。VEGF-A 也是一种血管扩张剂并增加微血管通透性^{[1][2][3][4][5]}。

The cell proliferation assay using human umbilical vein endothelial cells has an ED50 value of less than 5 ng/ml; corresponding to a specific activity of > 2.0 × 10⁵ IU/mg.

Mouse

E. coli

Tag Free

Q00731-3 (A27-R146)

22339  [NCBI]

APTTEGEQKSHEVIKFMDVYQRSYCRPIETLVDIFQEYPDEIEYIFKPSCVPLMRCAGCCNDEALECVPTSESNITMQIMRIKPHQSQHIGEMSFLQHSRCECRPKKDRTKPEKCDKPRR

Approximately 28.4 kDa (Disulfide-linked homodimer)

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg; determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.