VEGF121 蛋白, Human (120 a.a)

VEGF121 Protein, Human (120 a.a), a VEGF isoform splice variant, cannot bind to heparin. VEGF121 Protein is predictor for survival in activated B-cell-like diffuse large B-cell lymphoma and is related to an immune response gene signature conserved in cancers.

VEGF-121 缺乏肝素结合能力，需要细胞表面硫酸乙酰肝素才能与人黑色素瘤细胞的 VEGF 受体有效结合^[1]。此外，在体外血管生成模型中，VEGF-121 和 VEGF 165 通过血管内皮生长因子受体 2 调节血管直径。VEGF-121 是活化 B 细胞样弥漫性大 B 细胞淋巴瘤生存的预测因子，并且与癌症中保守的免疫反应基因特征相关^[2][3]。此外，VEGF-121 血浆水平是复发性胶质母细胞瘤抗血管生成治疗反应的生物标志物^[4]。

1. The ED₅₀ is ≤7.548 ng/mL as measured by HUVEC cells, corresponding to a specific activity of ≥1.32 × 10⁵ units/mg.
2. Immobilized Human VEGF 121 at 2 μg/mL (100 μl/well) can bind Human VEGFR2-Fc.The ED₅₀ of Human VEGFR2-Fc is ≤78 ng/mL.

• 
  Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED₅₀ for this effect is 7.474 ng/mL, corresponding to a specific activity is 1.34×10⁵ units/mg.

Human

E. coli

Tag Free

P15692-9 (P28-R147)

7422  [NCBI]

PMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQEKCDKPRR

Approximately 17 kDa under reduced condition.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS or PBS, pH 7.4, 8% trehalose.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Cohen T, et al. VEGF121, a vascular endothelial growth factor (VEGF) isoform lacking heparin binding ability, requires cell-surface heparan sulfates for efficient binding to the VEGF receptors of human melanoma cells. J Biol Chem. 1995 May 12;270(19):11322-6.  [Content Brief]

  [2]. Broséus J, et al. VEGF121, is predictor for survival in activated B-cell-like diffuse large B-cell lymphoma and is related to an immune response gene signature conserved in cancers. Oncotarget. 2017 Jul 19;8(53):90808-90824.  [Content Brief]

  [3]. Nakatsu MN, et al. VEGF(121) and VEGF(165) regulate blood vessel diameter through vascular endothelial growth factor receptor 2 in an in vitro angiogenesis model. Lab Invest. 2003 Dec;83(12):1873-85.  [Content Brief]

  [4]. Martini M, et al. VEGF-121 plasma level as biomarker for response to anti-angiogenetic therapy in recurrent glioblastoma. BMC Cancer. 2018 May 10;18(1):553.  [Content Brief]