VEGFR-1 蛋白, Rat (HEK293, His)

VEGFR-1 protein is a tyrosine protein kinase receptor for VEGFA, VEGFB, and PGF and is critical for embryonic vasculature development, angiogenesis, cell survival, migration, macrophage function, chemotaxis, and cancer invasion. It actively regulates postnatal retinal vitreous vascular degeneration and may act as a negative regulator of embryonic angiogenesis. VEGFR-1 Protein, Rat (HEK293, His) is the recombinant rat-derived VEGFR-1 protein, expressed by HEK293 , with C-His labeled tag. The total length of VEGFR-1 Protein, Rat (HEK293, His) is 736 a.a., with molecular weight of ~110-130 kDa.

VEGFR-1 蛋白是一种酪氨酸蛋白激酶，作为 VEGFA、VEGFB 和 PGF 的细胞表面受体，在胚胎脉管系统发育、血管生成调节、细胞存活、迁移、巨噬细胞功能、趋化性和癌症中发挥着至关重要的作用细胞侵袭。它作为出生后视网膜玻璃样血管退化的正调节剂，并且可以通过抑制内皮细胞的过度增殖作为胚胎血管生成的负调节剂。在成年期，VEGFR-1可以促进内皮细胞增殖、存活和血管生成，其在细胞增殖中的功能具有细胞类型特异性。它促进 PGF 介导的内皮细胞和某些癌细胞类型的增殖，但不促进正常成纤维细胞的增殖。凭借对 VEGFA 的高亲和力和相对较低的蛋白激酶活性，VEGFR-1 可通过限制游离 VEGFA 并阻止其与 KDR 结合，充当 VEGFA 信号转导的负调节因子。它通过与 KDR 形成异二聚体来调节 KDR 信号传导。配体结合激活多个信号级联，包括 PLCG 介导的二酰甘油和肌醇 1,4,5-三磷酸的产生、蛋白激酶 C 激活、PIK3R1 磷酸化（导致磷脂酰肌醇 3-激酶激活）以及随后的下游信号传导途径。VEGFR-1 还介导 MAP 激酶和 AKT1 信号通路的激活，磷酸化 SRC、YES1、PLCG 和潜在的 CBL，并促进 AKT1 和 PTK2/FAK1 磷酸化，突出其在调节复杂细胞过程中的多样化和不可或缺的作用。

Measured by its binding ability in a functional ELISA. Immobilized Human VEGF165 at 0.1 μg/mL(100 μL/well) can bind biotinylated Rat VEGFR-1. The ED₅₀ for this effect is 76.55 ng/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized Human VEGF165 at 0.1 μg/mL (100 μL/well) can bind biotinylated Rat VEGFR-1. The ED₅₀ for this effect is 76.55 ng/mL.

Rat

HEK293

C-His

P53767 (Y23-E758)

54251  [NCBI]

YCSGSKLKGPELSLKGTQHVMQAGQTLFLKCRGEAAHSWSLPTTVSQEDKKLSVTRSACGRNNRQFCSTLTLNMAQANHTGLYSCRYLPKSTSKEKKMESAIYIFVSDAGSPFIEMHSDIPKLVHMTEGRELIIPCRVTSPNITVTLKKFPFDALTPDGQRIAWDSRRGFIIANATYKEIGLLTCEATVNGHLYQTSYLTHRQTNTILDVQISPPSPVRFLRGQTLVLNCTVTTDLNTRVQMSWNYPGKATKRASIRQRIDQSNPHSNVFHSVLKINNVESRDKGLYTCRVKSGSSFRTFNTSVHVYEKGFISVKHRKQQVQETIAGKRSHRLSMKVKAFPSPEVVWLKDGVPATEKSARYSVHGYSLIIKDVTAEDAGDYTILLGIKQSKLFRNLTATLIVNVKPQIYEKSVSSLPSPPLYPLGSRQVLTCTVYGIPQPTIKWLWHPCHYNHSKERNDFCFGSEESFILDSSSNIGNRIEGITQRMMVIEGTNKTVSTLVVADSRTPGSYSCKAFNKIGTVERDIRFYVTDVPNGFHVSLEKIPTEGEDLKLSCVVSKFLYRDITWILLRTVNNRTMHHSISKQKMATTQDYSITLNLVIKNVSLEDSGTYACRARNIYTGEEILRKTEVLVRDLEAPLLLQNLSDHEVSISGSTTLDCQARGVPAPQITWFKNNHKIQQEPGIILGPGNSTLFIERVTEEDEGVYRCRATNQKGVVESSAYLTVQGTSDKSNLE

Approximately 110-130 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.